Williams A J, Coates P J, Lowe D G, McLean C, Gale E A
Department of Diabetes and Metabolism, St. Bartholomew's Hospital, London, UK.
Histopathology. 1992 Sep;21(3):215-23. doi: 10.1111/j.1365-2559.1992.tb00379.x.
An affinity purified antibody to fragment 14-29 of islet amyloid polypeptide (IAPP) has been prepared. This antibody, which does not cross-react with the related molecule calcitonin gene-related peptide, was used to investigate immunochemically the presence of IAPP in normal and neoplastic human pancreatic endocrine tissue. The pattern of IAPP staining in normal pancreas mirrors that of insulin, although slight differences were observed. In neoplastic tissue, IAPP was found in 16 out of 19 tumours that were positive for insulin, and was absent from one tumour negative for insulin. In some cases there were differences in the staining patterns of IAPP and insulin. These results suggest that the synthesis and secretion of IAPP and insulin are not inter-dependent and support the concept that IAPP has a discrete biological function. Islet amyloid polypeptide was found in six out of six insulinoma amyloid deposits, suggesting that the peptide is an invariable component of these deposits. Over-expression of IAPP, with aberrant processing and/or secretion, may be the causative factor for amyloid deposition in insulinomas and in the islets of type 2 (non-insulin dependent) diabetic patients. Investigation of patients with insulinomas and of insulin cells in culture and tissue sections may help to clarify the biological function of IAPP.
已制备出一种针对胰岛淀粉样多肽（IAPP）14 - 29片段的亲和纯化抗体。该抗体与相关分子降钙素基因相关肽无交叉反应，用于免疫化学研究IAPP在正常和肿瘤性人类胰腺内分泌组织中的存在情况。正常胰腺中IAPP的染色模式与胰岛素相似，不过也观察到了细微差异。在肿瘤组织中，19个胰岛素阳性肿瘤中有16个发现了IAPP，1个胰岛素阴性肿瘤中未发现IAPP。在某些情况下，IAPP和胰岛素的染色模式存在差异。这些结果表明，IAPP和胰岛素的合成与分泌并非相互依赖，支持IAPP具有独立生物学功能的概念。在6个胰岛素瘤淀粉样沉积物中均发现了胰岛淀粉样多肽，表明该肽是这些沉积物的恒定成分。IAPP的过度表达以及异常加工和/或分泌可能是胰岛素瘤和2型（非胰岛素依赖型）糖尿病患者胰岛中淀粉样沉积的致病因素。对胰岛素瘤患者以及培养物和组织切片中的胰岛素细胞进行研究，可能有助于阐明IAPP的生物学功能。
