HLA-DP区域的复杂性：限制性片段长度多态性分析与血小板分型及寡核苷酸分型的比较

Complexity of the HLA-DP region: RFLP analysis versus PLT typing and oligotyping.

作者信息

Dormoy A, Urlacher A, Tongio M M

机构信息

Regional Center for Blood Transfusion, Strasbourg, France.

出版信息

Hum Immunol. 1992 May;34(1):39-46. doi: 10.1016/0198-8859(92)90083-y.

DOI:10.1016/0198-8859(92)90083-y

PMID:1356955

Abstract

A total of 84 individuals were DP typed in parallel with the restriction fragment length polymorphism (RFLP) analysis and the primed lymphocyte test (PLT). Whereas 80% of the cases gave concordant results, the other 20% showed discrepancies for one of the two alleles carried by the typed individuals. Oligotyping, PCR-RFLP and sequencing confirmed the results found by PLT. The 20% discordant results obtained with RFLP led us to conclude that RFLP typing cannot replace PLT typing. From a more general point of view, the RFLP analysis revealed the DP region to be more complex than expected since for each given PLT DP defined specificity, more than one RFLP DP haplotype could be determined. These were possibly induced by crossing-overs or gene conversion events.

摘要

共有84人同时进行了DP分型，并与限制性片段长度多态性（RFLP）分析和启动淋巴细胞试验（PLT）同步进行。虽然80%的病例结果一致，但另外20%的病例在被分型个体携带的两个等位基因中的一个上出现了差异。寡核苷酸分型、PCR-RFLP和测序证实了PLT所获得的结果。RFLP获得的20%不一致结果使我们得出结论，RFLP分型不能替代PLT分型。从更一般的角度来看，RFLP分析显示DP区域比预期的更复杂，因为对于每个给定的PLT DP定义特异性，可以确定不止一种RFLP DP单倍型。这些可能是由交叉或基因转换事件引起的。