Maldonato A, Trueheart P A, Renold A E, Sharp G W
Diabetologia. 1976 Oct;12(5):471-81. doi: 10.1007/BF01219511.
Proinsulin synthesis, insulin release and intracellular ATP concentrations were measured in isolated rat islets of Langerhans under control conditions in vitro incubation and after treatment with several concentrations of streptozotocin for different periods of time. It was found that streptozotocin inhibited proinsulin synthesis, as well as insulin release, in a time and concentration dependent manner. The characteristics of the inhibition of these two processes were similar in general terms, but one dissimilarity was noted, i.e. after 60 min exposure to a high concentration of streptozotocin, proinsulin synthesis was inhibited more than insulin release. ATP content was reduced by high concentrations of streptozotocin, but it was found that proinsulin synthesis and insulin release could be inhibited without any effect on ATP content by a low (6.22 mM) concentration of streptozotocin. The effect of streptozotocin on proinsulin synthesis was judged to be the result of a target specificity for the B-cell rather than a specific effect on proinsulin relative to total protein synthesis.
在体外培养的对照条件下以及用几种浓度的链脲佐菌素处理不同时间段后，对分离的大鼠胰岛进行胰岛素原合成、胰岛素释放和细胞内ATP浓度的测定。结果发现，链脲佐菌素以时间和浓度依赖性方式抑制胰岛素原合成以及胰岛素释放。这两个过程的抑制特征总体上相似，但也注意到一个不同之处，即暴露于高浓度链脲佐菌素60分钟后，胰岛素原合成比胰岛素释放受到的抑制更明显。高浓度链脲佐菌素会降低ATP含量，但发现低浓度（6.22 mM）的链脲佐菌素可抑制胰岛素原合成和胰岛素释放，而对ATP含量没有任何影响。链脲佐菌素对胰岛素原合成的作用被认为是其对B细胞具有靶标特异性的结果，而非相对于总蛋白质合成对胰岛素原的特定作用。
