一种血清补体的连续测定方法。
A method for serial determinations of serum complement.
作者信息
WALTON K W, ELLIS H A
出版信息
Immunology. 1958 Jul;1(3):224-33.
A method is described for making serial estimations of serum complement. Titrations were made in a standardized haemolytic system and the 50 per cent haemolysis end point obtained by relating the probit percentage haemolysis to the reciprocal of the corresponding serum dilution. Possible variable factors such as changes in H and red cell concentration were studied and it was found that the most important source of error arose from the use of different batches of sheep red cells. This was overcome by running a lyophilized standard reference serum in parallel with all titrations and expressing the unknown sera in terms of this standard. Observations were then made on a group of selected subjects to form the basis for a study of changes in patients suffering from renal disease.
本文描述了一种进行血清补体系列测定的方法。在标准化溶血系统中进行滴定，并通过将概率溶血百分比与相应血清稀释度的倒数相关联来获得50%溶血终点。研究了诸如H和红细胞浓度变化等可能的可变因素，发现最重要的误差来源是使用不同批次的绵羊红细胞。通过在所有滴定中同时运行冻干标准参考血清并根据该标准表示未知血清来克服这一问题。然后对一组选定的受试者进行观察，为研究肾病患者的变化奠定基础。
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