Sano T, Imura R, Morishita Y, Matsuda Y, Yamada K
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Life Sci. 1992;51(18):1445-51. doi: 10.1016/0024-3205(92)90539-2.
HS-142-1, a novel polysaccharide, of microbial origin had been characterized as a specific antagonist of guanylyl cyclase-linked atrial natriuretic peptide (ANP) receptors (ANP-GC receptor) in bovine adrenal cortex. The effect of HS-142-1 on ANP receptors of rat glomeruli were examined. HS-142-1 blocked rat ANP (r-ANP)-stimulated cGMP production in a concentration-dependent manner, although it caused only slight inhibition in the specific binding of [125I]-rANP to the glomeruli where only a small portion of the binding sites are coupled to guanylyl cyclase. HS-142-1 recognized the 135K ANP receptor which is thought to be ANP-GC receptors but did not recognized 60K receptor, guanylyl cyclase-free type from affinity cross-linking studies with glomerular membranes. These results indicate that HS-142-1 is a specific antagonist for the ANP-GC receptor in rat glomeruli, and that it will be a powerful tool for understanding the physiological roles of ANP in renal responses.
HS - 142 - 1是一种源自微生物的新型多糖，已被鉴定为牛肾上腺皮质中鸟苷酸环化酶连接型心钠素（ANP）受体（ANP - GC受体）的特异性拮抗剂。研究了HS - 142 - 1对大鼠肾小球ANP受体的作用。HS - 142 - 1以浓度依赖性方式阻断大鼠ANP（r - ANP）刺激的cGMP生成，尽管它对[125I] - rANP与肾小球的特异性结合仅产生轻微抑制，而在肾小球中只有一小部分结合位点与鸟苷酸环化酶偶联。通过与肾小球膜的亲和交联研究，HS - 142 - 1识别被认为是ANP - GC受体的135K ANP受体，但不识别60K受体（无鸟苷酸环化酶型）。这些结果表明，HS - 142 - 1是大鼠肾小球中ANP - GC受体的特异性拮抗剂，并且它将成为理解ANP在肾脏反应中的生理作用的有力工具。
