H-2 I类Ld抗原在CD8⁺和CD4⁺T细胞中阳性选择不同的TCR Vβ基因产物。

H-2 class I Ld antigen positively selects different TCR V beta gene products in CD8+ and CD4+ T cells.

作者信息

Tscherning T, Reimann J, Claesson M H

机构信息

Department of Medical Anatomy A, University of Copenhagen, Denmark.

出版信息

Scand J Immunol. 1992 Oct;36(4):617-9. doi: 10.1111/j.1365-3083.1992.tb03230.x.

DOI:10.1111/j.1365-3083.1992.tb03230.x

PMID:1357740

Abstract

Expression of CD4+ or CD8+ determinants and ten different TCR V beta genes was analysed by two-colour flow cytometry in lymph node cells (LNC) of BALB/c and dm2 (a BALB/c Ld loss mutant) mice. TCR V beta 14 expression of CD8+ T cells and TCR V beta 7 and 14 of CD4+ T Cells were significantly more frequent in BALB/c than in dm2 LNC, whereas no differences were observed in the frequency distribution of TCR V beta+ CD4-CD8- double negative LNC from BALB/c versus dm2 mice. These results represent the first published evidence for positive selection of particular TCR V beta genes by individual MHC class I molecules in non-manipulated mice.

摘要

通过双色流式细胞术分析了BALB/c和dm2（一种BALB/c Ld缺失突变体）小鼠淋巴结细胞（LNC）中CD4⁺或CD8⁺决定簇以及十种不同的TCR Vβ基因的表达情况。BALB/c小鼠LNC中CD8⁺T细胞的TCR Vβ14表达以及CD4⁺T细胞的TCR Vβ7和Vβ14表达明显比dm2小鼠LNC中的更频繁，而BALB/c小鼠与dm2小鼠的TCR Vβ⁺CD4⁻CD8⁻双阴性LNC的频率分布未观察到差异。这些结果代表了在未处理的小鼠中，特定MHC I类分子对特定TCR Vβ基因进行阳性选择的首个已发表证据。