人类免疫缺陷病毒（HIV）相关疾病的进展与由限制性片段长度多态性定义的人类白细胞抗原（HLA）DQ和DR等位基因有关。

Progression of HIV-related disease is associated with HLA DQ and DR alleles defined by restriction fragment length polymorphisms.

作者信息

Donald J A, Rudman K, Cooper D W, Baumgart K W, Garsia R J, Gatenby P A, Rickard K A

机构信息

School of Biological Sciences, Macquarie University, Sydney, Australia.

出版信息

Tissue Antigens. 1992 May;39(5):241-8. doi: 10.1111/j.1399-0039.1992.tb01942.x.

DOI:10.1111/j.1399-0039.1992.tb01942.x

PMID:1357776

Abstract

A cohort of 139 hemophiliacs was typed for HLA D region genes by means of restriction fragment length polymorphisms (RFLPs) detected by HLA DQ and DR gene probes. Disease progression was studied in the 65 HIV antibody-positive patients, who were infected by contaminated clotting factor before 1985. Strong associations were found between disease progression in HIV-infected patients and allelic DNA fragments revealed by a DQ alpha cDNA probe. A 5.5 kb fragment was reduced in frequency and a 4.6 kb fragment increased in frequency (p less than 0.005) in the faster progressing group, as measured both by development of CDC Category IV clinical symptoms and CD4 number less than 200 x 10(6)/l. These results correlate with DR types deduced from the RFLP patterns revealed by DR beta and DQ alpha gene probes. A decrease in DR4 and an increase in both DR5 and the DR3 subtype found in the A1 B8 DR3 haplotype were associated with disease progression (p less than 0.05).

摘要

通过HLA DQ和DR基因探针检测到的限制性片段长度多态性（RFLP），对139名血友病患者进行了HLA D区域基因分型。对65名HIV抗体阳性患者的疾病进展情况进行了研究，这些患者在1985年之前因受污染的凝血因子而感染。在HIV感染患者的疾病进展与DQα cDNA探针揭示的等位基因DNA片段之间发现了强烈关联。在进展较快的组中，通过CDC IV类临床症状的出现和CD4细胞数低于200×10⁶/L来衡量，5.5 kb片段的频率降低，4.6 kb片段的频率增加（p<0.005）。这些结果与由DRβ和DQα基因探针揭示的RFLP模式推断出的DR类型相关。在疾病进展中，发现DR4减少，而在A1 B8 DR3单倍型中发现的DR5和DR3亚型均增加（p<0.05）。