Valiante N M, Rengaraju M, Trinchieri G
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Cell Immunol. 1992 Nov;145(1):187-98. doi: 10.1016/0008-8749(92)90322-g.
We have previously used human Epstein Barr virus (EBV)-transformed B lymphoblastoid cell lines for the identification and purification of a novel cytokine, natural killer cell stimulatory factor (NKSF/IL-12), that has pleiotropic effects on human lymphocytes. B cell lines are also routinely employed as feeder cells for the culture of T and natural killer (NK) cells. In this report we describe the ability of two NKSF/IL-12 producing B cell lines (RPMI-8866 and Cess) and two nonproducing lines (Raji and Daudi) to stimulate the proliferation of T and NK cells in 8-day PBL cultures. We demonstrate, using an anti-NKSF/IL-12 neutralizing monoclonal antibody, that the endogenous production of NKSF/IL-12 in these cultures can significantly enhance the proliferation and cytotoxic activity of T and NK cells. We also report that the addition of exogenous rNKSF/IL-12 can greatly increase the number of T and NK cells obtained from the cultures following stimulation by the B cell lines. Aside from the possible practical applications, the enhanced proliferation of T and NK cells consistently observed in the presence of endogenously produced NKSF/IL-12 or exogenously added rNKSF/IL-12 in this system may further our understanding of the role of this cytokine during an in vivo immune response.
我们之前曾使用人爱泼斯坦-巴尔病毒（EBV）转化的B淋巴母细胞系来鉴定和纯化一种新型细胞因子，即自然杀伤细胞刺激因子（NKSF/IL-12），它对人淋巴细胞具有多效性作用。B细胞系也经常被用作T细胞和自然杀伤（NK）细胞培养的饲养细胞。在本报告中，我们描述了两种产生NKSF/IL-12的B细胞系（RPMI-8866和Cess）以及两种不产生该因子的细胞系（Raji和Daudi）在8天外周血淋巴细胞（PBL）培养物中刺激T细胞和NK细胞增殖的能力。我们使用抗NKSF/IL-12中和单克隆抗体证明，这些培养物中内源性产生的NKSF/IL-12可显著增强T细胞和NK细胞的增殖及细胞毒性活性。我们还报告称，添加外源性重组NKSF/IL-12可极大增加B细胞系刺激后从培养物中获得的T细胞和NK细胞数量。除了可能的实际应用外，在该系统中，在存在内源性产生的NKSF/IL-12或外源性添加的重组NKSF/IL-12时持续观察到的T细胞和NK细胞增殖增强，可能会增进我们对这种细胞因子在体内免疫反应中作用的理解。
