阿片类药物和α2-肾上腺素能受体激动剂对心脏感觉神经元的抑制作用对百日咳毒素不敏感。

The inhibitory effect of opioid and alpha 2-adrenoceptor agonists on cardiac sensory neurones is pertussis toxin-insensitive.

作者信息

Amerini S, Rubino A, Mantelli L, Filippi S, Ledda F

机构信息

Department of Pharmacology, University of Florence, Italy.

出版信息

Eur J Pharmacol. 1992 Jun 17;216(3):445-8. doi: 10.1016/0014-2999(92)90444-9.

DOI:10.1016/0014-2999(92)90444-9

PMID:1358630

Abstract

The role of pertussis toxin-sensitive G proteins on the alpha 2-adrenoceptor and mu-opioid receptor-mediated inhibition of the efferent function of capsaicin-sensitive neurones was investigated in guinea-pig atria pretreated with guanethidine. In the presence of atropine, CGP 20712A (2-hydroxy-5-(2-[hydroxy-3-(4-[(1-methyl- 4-trifluormethyl)1H-imidazol-2-yl]-phenoxy)propyl]aminoethoxyl+ ++)-benzamide) and prazosin, [D-Ala2,NMe-Phe4,Gly5-ol]enkephalin (DAGO, 0.1-3 microM) and 2-amino-6-allyl-5,6,7,8-tetrahydro-4H-thiazolo(4,5-d)azepine (BHT 920, 0.01-1 microM) reduced the positive inotropic effect induced by transmural stimulation of preparations obtained from control and from pertussis toxin-treated animals. These results suggest that pertussis toxin-sensitive G proteins are not involved in the inhibitory regulation of the efferent function of capsaicin-sensitive nerve terminals in cardiac tissue induced by alpha 2 and opioid receptor stimulation.

摘要

在经胍乙啶预处理的豚鼠心房中，研究了百日咳毒素敏感的G蛋白在α2 -肾上腺素能受体和μ-阿片受体介导的对辣椒素敏感神经元传出功能抑制中的作用。在阿托品、CGP 20712A（2 -羟基-5 -（2 - [羟基-3 -（4 - [（1 -甲基-4 -三氟甲基）1H -咪唑-2 -基] -苯氧基）丙基]氨基乙氧基）-苯甲酰胺）和哌唑嗪存在的情况下，[D - Ala2，NMe - Phe4，Gly5 - ol]脑啡肽（DAGO，0.1 - 3 microM）和2 -氨基-6 -烯丙基-5,6,7,8 -四氢-4H -噻唑并（4,5 - d）氮杂卓（BHT 920，0.01 - 1 microM）降低了由对照动物和百日咳毒素处理动物制备物的透壁刺激所诱导的正性肌力作用。这些结果表明，百日咳毒素敏感的G蛋白不参与α2和阿片受体刺激所诱导的心脏组织中辣椒素敏感神经末梢传出功能的抑制调节。