• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

淋病奈瑟菌菌毛（菌毛）在铜绿假单胞菌中的产生。

Production of Neisseria gonorrhoeae pili (fimbriae) in Pseudomonas aeruginosa.

作者信息

Hoyne P A, Haas R, Meyer T F, Davies J K, Elleman T C

机构信息

Division of Biomolecular Engineering, Commonwealth Scientific and Industrial Research Organisation, Parkville, Victoria, Australia.

出版信息

J Bacteriol. 1992 Nov;174(22):7321-7. doi: 10.1128/jb.174.22.7321-7327.1992.

DOI:10.1128/jb.174.22.7321-7327.1992
PMID:1358873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207427/
Abstract

Pseudomonas aeruginosa K/2PfS, when transformed with an expression plasmid harboring the pilin gene (pilE1) of Neisseria gonorrhoeae MS11, was able to express and assemble gonococcal pilin monomers into surface-associated pili, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and immunoelectron microscopy. Concomitant with the expression of gonococcal pili in P. aeruginosa was the virtual loss of production of P. aeruginosa K/2PfS pili normally associated with the host cell.

摘要

用携带淋病奈瑟菌MS11菌毛蛋白基因（pilE1）的表达质粒转化铜绿假单胞菌K/2PfS后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹和免疫电子显微镜判断，该菌能够表达淋病奈瑟菌菌毛单体并将其组装成与表面相关的菌毛。在铜绿假单胞菌中表达淋病奈瑟菌菌毛的同时，通常与宿主细胞相关的铜绿假单胞菌K/2PfS菌毛的产生实际上消失了。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/82908bb22932/jbacter00088-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/bcd5d7572463/jbacter00088-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/6e6e9a02641f/jbacter00088-0263-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/82908bb22932/jbacter00088-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/bcd5d7572463/jbacter00088-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/6e6e9a02641f/jbacter00088-0263-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74ed/207427/82908bb22932/jbacter00088-0264-a.jpg

相似文献

1
Production of Neisseria gonorrhoeae pili (fimbriae) in Pseudomonas aeruginosa.淋病奈瑟菌菌毛（菌毛）在铜绿假单胞菌中的产生。
J Bacteriol. 1992 Nov;174(22):7321-7. doi: 10.1128/jb.174.22.7321-7327.1992.
2
Cryoelectron Microscopy Reconstructions of the Pseudomonas aeruginosa and Neisseria gonorrhoeae Type IV Pili at Sub-nanometer Resolution.铜绿假单胞菌和淋病奈瑟菌IV型菌毛亚纳米分辨率的冷冻电子显微镜重建
Structure. 2017 Sep 5;25(9):1423-1435.e4. doi: 10.1016/j.str.2017.07.016.
3
Expression of multiple types of N-methyl Phe pili in Pseudomonas aeruginosa.
Mol Microbiol. 1987 Nov;1(3):377-80. doi: 10.1111/j.1365-2958.1987.tb01945.x.
4
Pilin expression and processing in pilus mutants of Neisseria gonorrhoeae: critical role of Gly-1 in assembly.淋病奈瑟菌菌毛突变体中菌毛蛋白的表达与加工：甘氨酸-1在组装中的关键作用
Mol Microbiol. 1991 Feb;5(2):279-87. doi: 10.1111/j.1365-2958.1991.tb02108.x.
5
Silent pilin genes of Neisseria gonorrhoeae MS11 and the occurrence of related hypervariant sequences among other gonococcal isolates.淋病奈瑟菌MS11的沉默菌毛蛋白基因及其他淋球菌分离株中相关高变序列的出现情况。
Mol Microbiol. 1992 Jan;6(2):197-208. doi: 10.1111/j.1365-2958.1992.tb02001.x.
6
On the role of pili in transformation of Neisseria gonorrhoeae.菌毛在淋病奈瑟菌转化中的作用
J Gen Microbiol. 1984 Dec;130(12):3165-73. doi: 10.1099/00221287-130-12-3165.
7
Neisseria meningitidis C114 contains silent, truncated pilin genes that are homologous to Neisseria gonorrhoeae pil sequences.脑膜炎奈瑟菌C114含有沉默的、截短的菌毛蛋白基因，这些基因与淋病奈瑟菌菌毛序列同源。
J Bacteriol. 1988 Apr;170(4):1691-7. doi: 10.1128/jb.170.4.1691-1697.1988.
8
Neisseria gonorrhoeae PilC expression provides a selective mechanism for structural diversity of pili.淋病奈瑟菌PilC表达为菌毛的结构多样性提供了一种选择机制。
Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3204-8. doi: 10.1073/pnas.89.8.3204.
9
PilC of Neisseria meningitidis is involved in class II pilus formation and restores pilus assembly, natural transformation competence and adherence to epithelial cells in PilC-deficient gonococci.脑膜炎奈瑟菌的PilC参与II型菌毛形成，并能恢复PilC缺陷型淋球菌的菌毛组装、自然转化能力以及对上皮细胞的黏附。
Mol Microbiol. 1997 Mar;23(5):879-92. doi: 10.1046/j.1365-2958.1997.2631630.x.
10
Molecular principles of antigenic variation in Neisseria gonorrhoeae.淋病奈瑟菌抗原变异的分子原理
Antonie Van Leeuwenhoek. 1987;53(6):431-4. doi: 10.1007/BF00415498.

引用本文的文献

1
Identification and Characterization of Type IV Pili as the Cellular Receptor of Broad Host Range Bacteriophages DLP1 and DLP2.鉴定和表征 IV 型菌毛作为广谱宿主噬菌体 DLP1 和 DLP2 的细胞受体。
Viruses. 2018 Jun 20;10(6):338. doi: 10.3390/v10060338.
2
A Pilin Region Affecting Host Range of the RNA Phage, PP7.影响RNA噬菌体PP7宿主范围的菌毛区域
Front Microbiol. 2018 Feb 16;9:247. doi: 10.3389/fmicb.2018.00247. eCollection 2018.
3
A Geobacter sulfurreducens strain expressing pseudomonas aeruginosa type IV pili localizes OmcS on pili but is deficient in Fe(III) oxide reduction and current production.

本文引用的文献

1
Cloning and expression in Escherichia coli of the gene encoding the structural subunit of Bacteroides nodosus fimbriae.诺氏拟杆菌菌毛结构亚基编码基因在大肠杆菌中的克隆与表达。
J Bacteriol. 1984 Nov;160(2):748-54. doi: 10.1128/jb.160.2.748-754.1984.
2
Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence.淋病奈瑟菌菌毛基因：染色体组织与DNA序列
Proc Natl Acad Sci U S A. 1984 Oct;81(19):6110-4. doi: 10.1073/pnas.81.19.6110.
3
Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement.淋病奈瑟菌菌毛的表达涉及染色体重排。
表达绿脓假单胞菌 IV 型菌毛的脱硫肠状菌菌株将 OmcS 定位于菌毛上，但在氧化铁还原和电流产生方面存在缺陷。
Appl Environ Microbiol. 2014 Feb;80(3):1219-24. doi: 10.1128/AEM.02938-13. Epub 2013 Dec 2.
4
Novel proteins that modulate type IV pilus retraction dynamics in Pseudomonas aeruginosa.调节铜绿假单胞菌IV型菌毛回缩动力学的新型蛋白质。
J Bacteriol. 2008 Nov;190(21):7022-34. doi: 10.1128/JB.00938-08. Epub 2008 Sep 5.
5
Pseudomonas aeruginosa Type IV pilus expression in Neisseria gonorrhoeae: effects of pilin subunit composition on function and organelle dynamics.铜绿假单胞菌IV型菌毛在淋病奈瑟菌中的表达：菌毛亚基组成对功能和细胞器动力学的影响。
J Bacteriol. 2007 Sep;189(18):6676-85. doi: 10.1128/JB.00407-07. Epub 2007 Jun 15.
6
Type II protein secretion in Pseudomonas aeruginosa: the pseudopilus is a multifibrillar and adhesive structure.铜绿假单胞菌中的II型蛋白质分泌：假菌毛是一种多纤维且具粘附性的结构。
J Bacteriol. 2003 May;185(9):2749-58. doi: 10.1128/JB.185.9.2749-2758.2003.
7
Analysis of the type IV fimbrial-subunit gene fimA of Xanthomonas hyacinthi: application in PCR-mediated detection of yellow disease in Hyacinths.风信子黄单胞菌IV型菌毛亚基基因fimA的分析：在PCR介导的风信子黄化病检测中的应用。
Appl Environ Microbiol. 2001 Feb;67(2):598-607. doi: 10.1128/AEM.67.2.598-607.2001.
8
PpdD type IV pilin of Escherichia coli K-12 can Be assembled into pili in Pseudomonas aeruginosa.大肠杆菌K-12的PpdD IV型菌毛蛋白可在铜绿假单胞菌中组装成菌毛。
J Bacteriol. 2000 Feb;182(3):848-54. doi: 10.1128/JB.182.3.848-854.2000.
9
Overexpression of Methanococcus voltae flagellin subunits in Escherichia coli and Pseudomonas aeruginosa: a source of archaeal preflagellin.沃氏甲烷球菌鞭毛蛋白亚基在大肠杆菌和铜绿假单胞菌中的过表达：古菌前鞭毛蛋白的一个来源。
J Bacteriol. 1999 Jul;181(14):4146-53. doi: 10.1128/JB.181.14.4146-4153.1999.
10
Roles of PilC and PilE proteins in pilus-mediated adherence of Neisseria gonorrhoeae and Neisseria meningitidis to human erythrocytes and endothelial and epithelial cells.PilC和PilE蛋白在淋病奈瑟菌和脑膜炎奈瑟菌菌毛介导的对人红细胞、内皮细胞及上皮细胞黏附中的作用。
Infect Immun. 1999 Feb;67(2):834-43. doi: 10.1128/IAI.67.2.834-843.1999.
Cell. 1982 Aug;30(1):45-52. doi: 10.1016/0092-8674(82)90010-1.
4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
The adsorption of Pseudomonas aeruginosa pilus-dependent bacteriophages to a host mutant with nonretractile pili.
Virology. 1974 Mar;58(1):149-63. doi: 10.1016/0042-6822(74)90150-0.
6
Cloning vectors derived from the Pseudomonas plasmid pVS1.源自假单胞菌质粒pVS1的克隆载体。
Gene. 1985;36(1-2):27-36. doi: 10.1016/0378-1119(85)90066-6.
7
Pilins of Bacteroides nodosus: molecular basis of serotypic variation and relationships to other bacterial pilins.结节拟杆菌菌毛蛋白：血清型变异的分子基础及其与其他细菌菌毛蛋白的关系
Microbiol Rev. 1988 Jun;52(2):233-47. doi: 10.1128/mr.52.2.233-247.1988.
8
Proteins that appear to be associated with pili in Neisseria gonorrhoeae.在淋病奈瑟菌中似乎与菌毛相关的蛋白质。
Infect Immun. 1988 Jul;56(7):1743-7. doi: 10.1128/iai.56.7.1743-1747.1988.
9
Expression of multiple types of N-methyl Phe pili in Pseudomonas aeruginosa.
Mol Microbiol. 1987 Nov;1(3):377-80. doi: 10.1111/j.1365-2958.1987.tb01945.x.
10
Release of soluble pilin antigen coupled with gene conversion in Neisseria gonorrhoeae.淋病奈瑟菌中可溶性菌毛抗原的释放与基因转换
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9079-83. doi: 10.1073/pnas.84.24.9079.