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幽门螺杆菌脲酶与一种GroEL类似物的结构比较。

Structural comparison of urease and a GroEL analog from Helicobacter pylori.

作者信息

Austin J W, Doig P, Stewart M, Trust T J

机构信息

Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.

出版信息

J Bacteriol. 1992 Nov;174(22):7470-3. doi: 10.1128/jb.174.22.7470-7473.1992.

DOI:10.1128/jb.174.22.7470-7473.1992
PMID:1358875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207446/
Abstract

Electron microscopy of purified protein preparations indicated that Helicobacter pylori urease consisted of circular particles that are 13 nm in diameter, some of which showed indications of threefold rotational symmetry. A GroEL analog of H. pylori (Hp60K) appeared as a disc-shaped molecule with a diameter similar to that of urease but possessed sevenfold rotational symmetry. In a side-view projection, Hp60K appeared as two or four discs stacked side by side.

摘要

纯化蛋白质制剂的电子显微镜检查表明，幽门螺杆菌尿素酶由直径为13nm的圆形颗粒组成，其中一些显示出三重旋转对称的迹象。幽门螺杆菌的一种GroEL类似物（Hp60K）呈现为盘状分子，其直径与尿素酶相似，但具有七重旋转对称。在侧视图投影中，Hp60K呈现为两个或四个并排堆叠的圆盘。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b68c/207446/f4d79b68dd41/jbacter00088-0412-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b68c/207446/024a6cffcd3e/jbacter00088-0411-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b68c/207446/f4d79b68dd41/jbacter00088-0412-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b68c/207446/024a6cffcd3e/jbacter00088-0411-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b68c/207446/f4d79b68dd41/jbacter00088-0412-a.jpg

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本文引用的文献

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A protein similar to Escherichia coli gro EL is present in Bacillus subtilis.一种与大肠杆菌gro EL相似的蛋白质存在于枯草芽孢杆菌中。
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Nucleotide sequence of two genes from Helicobacter pylori encoding for urease subunits.幽门螺杆菌编码脲酶亚基的两个基因的核苷酸序列。
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