采用带荧光检测的高效液相色谱法对牛视网膜酪氨酸羟化酶进行简单准确的测定。
Simple and accurate assay for tyrosine hydroxylase from bovine retina by high-performance liquid chromatography with fluorescence detection.
作者信息
Mandai M, Iwaki M, Honda Y
机构信息
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
出版信息
Ophthalmic Res. 1992;24(4):228-33. doi: 10.1159/000267172.
We present a simple and sensitive assay for tyrosine hydroxylase (TH) using high-performance liquid chromatography (HPLC) with a fluorescence detector which enabled us to study bovine retinal TH of a small quantity in crude retinal samples. The detection limit with this method was 1 pmol of dihydroxyphenylalanine (DOPA) enzymatically produced. Retinal TH exhibited the maximum activity at pH 6 to 6.3, and almost none at physiological pH of above 7, a tendency similar to that reported for striatal TH.
我们提出了一种使用配备荧光检测器的高效液相色谱法（HPLC）检测酪氨酸羟化酶（TH）的简单且灵敏的方法，该方法使我们能够研究粗视网膜样品中少量的牛视网膜TH。此方法的检测限为酶促产生的1皮摩尔二羟基苯丙氨酸（DOPA）。视网膜TH在pH 6至6.3时表现出最大活性，而在高于7的生理pH下几乎没有活性，这一趋势与纹状体TH的报道相似。
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