Mandai M, Iwaki M, Honda Y
Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
Ophthalmic Res. 1992;24(4):228-33. doi: 10.1159/000267172.
We present a simple and sensitive assay for tyrosine hydroxylase (TH) using high-performance liquid chromatography (HPLC) with a fluorescence detector which enabled us to study bovine retinal TH of a small quantity in crude retinal samples. The detection limit with this method was 1 pmol of dihydroxyphenylalanine (DOPA) enzymatically produced. Retinal TH exhibited the maximum activity at pH 6 to 6.3, and almost none at physiological pH of above 7, a tendency similar to that reported for striatal TH.
我们提出了一种使用配备荧光检测器的高效液相色谱法（HPLC）检测酪氨酸羟化酶（TH）的简单且灵敏的方法，该方法使我们能够研究粗视网膜样品中少量的牛视网膜TH。此方法的检测限为酶促产生的1皮摩尔二羟基苯丙氨酸（DOPA）。视网膜TH在pH 6至6.3时表现出最大活性，而在高于7的生理pH下几乎没有活性，这一趋势与纹状体TH的报道相似。
