Gromoll J, Gudermann T, Nieschlag E
Institute of Reproductive Medicine of the University, Münster, Germany.
Biochem Biophys Res Commun. 1992 Nov 16;188(3):1077-83. doi: 10.1016/0006-291x(92)91341-m.
Northern blot hybridization of human testicular poly (A)+ RNA to a human follicle stimulating hormone receptor probe revealed the existence of multiple mRNA transcripts. In order to investigate whether alternative splicing of the receptor occurs in the human testis we amplified the extracellular and the transmembrane domain of the human testicular follicle stimulating hormone receptor by reverse transcription polymerase chain reaction and subcloned the resulting DNA fragments. Sequence analysis of the recombinant clones revealed the existence of a truncated isoform of the human follicle stimulating hormone receptor which is spliced through a cassette exon mode without a change in the open reading frame, thereby deleting exon IX from the coding region of the receptor.
用人促卵泡激素受体探针与人睾丸多聚腺苷酸尾化RNA进行Northern印迹杂交，结果显示存在多种mRNA转录本。为了研究该受体的可变剪接是否在人睾丸中发生，我们通过逆转录聚合酶链反应扩增了人睾丸促卵泡激素受体的细胞外结构域和跨膜结构域，并将所得DNA片段进行亚克隆。重组克隆的序列分析显示，人促卵泡激素受体存在一种截短的异构体，它通过盒式外显子模式进行剪接，开放阅读框没有变化，从而从受体编码区缺失外显子IX。
