Ichikawa A, Shimamura K, Koyama Y
Department of Medical Technology, Tochigi Cancer Center.
Nihon Rinsho. 1992 Oct;50(10):2343-8.
Flow cytometric techniques have been developed for measurement of proliferating cell nuclear antigen (PCNA), which allows studies on the proliferative capacity of cells and tissues. PCNA-DNA dual staining procedures, for both fixed and unfixed cells, and analytical method by FCM are presented. We recommend performing either the fixed or unfixed method. Our studies using the MCF7 human breast adenocarcinoma cell line and the A549 human lung squamous cell carcinoma cell line, in the exponential growth phase, revealed that both ethanol and acetone were satisfactory fixatives, in contrast to methanol and paraformaldehyde, and PI with a concentration of 25 micrograms/ml was most suitable compared with 50 and 100 micrograms/ml.
流式细胞术已被开发用于测量增殖细胞核抗原（PCNA），这使得对细胞和组织的增殖能力进行研究成为可能。本文介绍了针对固定和未固定细胞的PCNA-DNA双重染色程序以及通过流式细胞仪的分析方法。我们建议采用固定或未固定方法中的任意一种。我们使用处于指数生长期的MCF7人乳腺腺癌细胞系和A549人肺鳞状细胞癌细胞系进行的研究表明，与甲醇和多聚甲醛相比，乙醇和丙酮都是令人满意的固定剂，并且与50和100微克/毫升相比，浓度为25微克/毫升的碘化丙啶（PI）最为合适。
