Baran V, Veselá J, Rehák P, Koppel J
Slovak Academy of Sciences, Institute of Animal Physiology, Kosice.
Reprod Nutr Dev. 1992;32(3):241-8. doi: 10.1051/rnd:19920303.
Immunolocalization of cyclin/PCNA (proliferating cell nuclear antigen) was performed with monoclonal antibody using immunogold methods on ultrathin cryosections of fertilized mouse eggs. Immunolabeling in pronuclei was checked 20, 22, 24 and 26 h after HCG injection. A relation between onset of pronuclei migration (early S-phase) and appearance of colloidal particle clusters was found. Afterwards, (mid S-phase) the increase of labelling and the localization of cyclin/PCNA were found throughout the pronuclei, except in the nucleolar bodies. Lower labelling appeared at the time of close reciprocal pronuclei contact (late S-phase). It is concluded that bulk and distribution of cyclin/PCNA in pronuclei is closely related to the progression of first interphase after fertilization.
使用免疫金法，用单克隆抗体对受精小鼠卵的超薄冷冻切片进行细胞周期蛋白/增殖细胞核抗原（PCNA）的免疫定位。在注射人绒毛膜促性腺激素（HCG）后20、22、24和26小时检查原核中的免疫标记。发现原核迁移开始（早期S期）与胶体颗粒簇的出现之间存在关联。之后，（中期S期）除核仁体外，在整个原核中发现标记增加和细胞周期蛋白/PCNA的定位。在原核紧密相互接触时（晚期S期）标记较低。得出的结论是，原核中细胞周期蛋白/PCNA的数量和分布与受精后第一次间期的进程密切相关。
