Lucchiari M A, Modolell M, Eichmann K, Pereira C A
Instituto Butantan, Laboratorio de Imunologia Viral, São Paulo, Brasil.
Immunobiology. 1992 Sep;185(5):475-82. doi: 10.1016/s0171-2985(11)80089-6.
The possible role of interferon-gamma (IFN-gamma) in the resistance of A/J mice to MHV3 infection was investigated. Monoclonal antibodies specific for IFN-gamma, CD4 and CD8 molecules were administered in vivo to deplete selectively the IFN-gamma synthesized or the appropriate subset of T cells. The animals were then infected with MHV3 and the course of infection was followed by studying different parameters, such as, the mortality, the virus growth in the tissues and the IFN-gamma synthesis in sera and peritoneal exudates. After MHV3 infection, a full resistance of control A/J mice was observed, in contrast to the high mortality rate observed among the depleted animals, where higher virus titers were found in different tissues. The IFN-gamma synthesis in sera and peritoneal exudates of depleted mice, after MHV3 infection, drastically decreased when compared to that detected in control mice. The data presented are consistent with the hypothesis that IFN-gamma plays an essential role in the resistance of A/J mice to MHV3 infection.
研究了γ干扰素（IFN-γ）在A/J小鼠抵抗MHV3感染中可能发挥的作用。体内给予针对IFN-γ、CD4和CD8分子的单克隆抗体，以选择性清除合成的IFN-γ或适当的T细胞亚群。然后用MHV3感染动物，并通过研究不同参数来跟踪感染过程，如死亡率、组织中的病毒生长以及血清和腹腔渗出液中的IFN-γ合成。感染MHV3后，观察到对照A/J小鼠具有完全抵抗力，与之形成对比的是，在被清除的动物中观察到高死亡率，且在不同组织中发现了更高的病毒滴度。与对照小鼠相比，感染MHV3后，被清除小鼠的血清和腹腔渗出液中的IFN-γ合成急剧下降。所呈现的数据与IFN-γ在A/J小鼠抵抗MHV3感染中起重要作用的假设一致。
