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采用时间分辨荧光免疫分析法检测自然疫源地采集的硬蜱中蜱传脑炎病毒

Detection of tick-borne encephalitis virus in ixodid ticks collected in natural foci by time-resolved fluoroimmunoassay.

作者信息

Pomelova V G, Lavrova N A, Gaidamovich S Y, Navolokin O V, Halonen P, Sokolova M V, Kharitonenkov I G

机构信息

Ivanovsky Institute of Virology of the U.S.S.R. AMS, Moscow.

出版信息

Acta Virol. 1992 May;36(3):260-8.

PMID:1360754
Abstract

Time-resolved fluoroimmunoassay (TR-FIA) was used for the first time for evaluation of infestation of ixodid ticks with tick-borne encephalitis virus. Comparison of TR-FIA results with those obtained in enzyme immunoassay and by virus isolation confirmed the high efficacy of the method in question. Positive results of TR-FIA coincided with the data of virus isolation in 83.6% cases, the level of false-negative results did not exceed 1.2%, the overall time consumption amounted to about 1.2 hr.

摘要

首次使用时间分辨荧光免疫分析(TR-FIA)评估硬蜱感染蜱传脑炎病毒的情况。将TR-FIA结果与酶免疫分析及病毒分离获得的结果进行比较,证实了该方法具有很高的效能。TR-FIA的阳性结果与病毒分离数据在83.6%的病例中相符,假阴性结果水平不超过1.2%,总耗时约为1.2小时。

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