Appel S H, Le W D, Tajti J, Haverkamp L J, Engelhardt J I
Department of Neurology, Baylor College of Medicine, Houston, TX 77030.
Ann Neurol. 1992 Oct;32(4):494-501. doi: 10.1002/ana.410320403.
To support a potential role for immune mechanisms in the destruction of substantia nigra (SN) neurons, guinea pigs were immunized with bovine mesencephalon containing SN neurons. After immunization no clinical signs of basal ganglia dysfunction appeared. However, pathological examination revealed evidence of neuronal damage in the SN in 8 of 17 guinea pigs immunized with bovine mesencephalon. No nigral pathology was noted in animals immunized with spinal cord gray matter or Freund's adjuvant alone. Accompanying the SN damage in mesencephalon-immunized guinea pigs was a 25% decrease in tyrosine hydroxylase activity in the SN and a 27% decrease in dopamine content in the striatum. Deposits of IgG were detected by immunohistochemical techniques in sections of SN from mesencephalon-immunized guinea pigs and in sections of human SN after exposure to serum from mesencephalon-immunized guinea pigs. These data document the antigenicity of SN and suggest the possibility that immune mechanisms can contribute to basal ganglia pathology.
为了支持免疫机制在黑质（SN）神经元破坏中可能发挥的作用，用含有SN神经元的牛中脑对豚鼠进行免疫。免疫后未出现基底神经节功能障碍的临床症状。然而，病理检查发现，在17只接受牛中脑免疫的豚鼠中，有8只的SN存在神经元损伤迹象。在用脊髓灰质或单独的弗氏佐剂免疫的动物中未发现黑质病变。在接受中脑免疫的豚鼠中，伴随SN损伤的是SN中酪氨酸羟化酶活性降低25%，纹状体中多巴胺含量降低27%。通过免疫组织化学技术在接受中脑免疫的豚鼠的SN切片以及暴露于接受中脑免疫的豚鼠血清后的人类SN切片中检测到IgG沉积。这些数据证明了SN的抗原性，并提示免疫机制可能导致基底神经节病变。
