Severina I S, Busygina O G
Biokhimiia. 1992 Sep;57(9):1330-6.
The effect of carnosine on activation of human platelet soluble guanylate cyclase has been studied in 105,000 g supernatants and partially purified haem-deficient enzyme preparations. In the 105,000 g supernatant carnosine (1 mM) inhibited (by about 70%) the enzyme activation caused by sodium nitroprusside. In partially purified haem-deficient guanylate cyclase preparations the inhibition of enzyme activation by sodium nitroprusside was 86%; further addition of carnosine had no effect on the enzyme activity. The strength of the activating effect of protoporphyrin IX on partially purified haem-deficient guanylate cyclase did not differ from that for the 105,000 g supernatant; this stimulating effect did not change after carnosine addition. A conclusion is drawn that the inhibiting effect of carnosine on the ability of guanylate cyclase to be activated by sodium nitroprusside is due to the dipeptide interaction with the guanylate cyclase haem.
已在105,000g上清液和部分纯化的血红素缺乏酶制剂中研究了肌肽对人血小板可溶性鸟苷酸环化酶激活的影响。在105,000g上清液中，肌肽（1mM）抑制（约70%）由硝普钠引起的酶激活。在部分纯化的血红素缺乏鸟苷酸环化酶制剂中，硝普钠对酶激活的抑制率为86%；进一步添加肌肽对酶活性无影响。原卟啉IX对部分纯化的血红素缺乏鸟苷酸环化酶的激活作用强度与105,000g上清液的激活作用强度无差异；添加肌肽后这种刺激作用未改变。得出的结论是，肌肽对鸟苷酸环化酶被硝普钠激活能力的抑制作用是由于二肽与鸟苷酸环化酶血红素的相互作用。
