• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Site-directed mutagenesis of conserved residues of Clostridium thermocellum endoglucanase CelC.

作者信息

Navas J, Béguin P

机构信息

Département des Biotechnologies, Institut Pasteur, Paris, France.

出版信息

Biochem Biophys Res Commun. 1992 Dec 15;189(2):807-12. doi: 10.1016/0006-291x(92)92274-2.

DOI:10.1016/0006-291x(92)92274-2
PMID:1361730
Abstract

Four conserved residues of Clostridium thermocellum endoglucanase CelC were replaced by site-directed mutagenesis. Proteins mutated in His-90, Asn-139 and Glu-140 showed strongly reduced activity, in agreement with predictions of sequence alignments. Mutations in Glu-140 did not result in any detectable change in Km, or apparent size, suggesting that Glu-140 is directly involved in catalysis. The pH optimum of the proteins carrying the Glu-140/Ala and Glu140/Gln mutations was lower than that of the wild type, whereas the activity vs. pH profile of Glu-140/Asp CelC was similar to that of the wild type, suggesting that Glu-140 may act as a proton donor.

摘要

相似文献

1
Site-directed mutagenesis of conserved residues of Clostridium thermocellum endoglucanase CelC.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):807-12. doi: 10.1016/0006-291x(92)92274-2.
2
Site-directed mutagenesis of essential carboxylic residues in Clostridium thermocellum endoglucanase CelD.
J Biol Chem. 1992 Mar 5;267(7):4472-8.
3
Properties conferred on Clostridium thermocellum endoglucanase CelC by grafting the duplicated segment of endoglucanase CelD.通过嫁接内切葡聚糖酶CelD的重复片段赋予热纤梭菌内切葡聚糖酶CelC的特性。
Protein Eng. 1993 Nov;6(8):947-52. doi: 10.1093/protein/6.8.947.
4
The catalytic domain of endoglucanase A from Clostridium cellulolyticum: effects of arginine 79 and histidine 122 mutations on catalysis.来自解纤维梭菌的内切葡聚糖酶A的催化结构域：精氨酸79和组氨酸122突变对催化作用的影响。
J Bacteriol. 1992 Jul;174(14):4677-82. doi: 10.1128/jb.174.14.4677-4682.1992.
5
Identification of a histidyl residue in the active center of endoglucanase D from Clostridium thermocellum.
J Biol Chem. 1991 Jun 5;266(16):10313-8.
6
Modification of catalytically important carboxy residues in endoglucanase D from Clostridium thermocellum.嗜热栖热放线菌内切葡聚糖酶D中具有催化重要性的羧基残基的修饰。
Biochem J. 1992 Jul 1;285 ( Pt 1)(Pt 1):319-24. doi: 10.1042/bj2850319.
7
The crystal structure of a family 5 endoglucanase mutant in complexed and uncomplexed forms reveals an induced fit activation mechanism.一个5族内切葡聚糖酶突变体的复合形式和非复合形式的晶体结构揭示了一种诱导契合激活机制。
J Mol Biol. 1996 Apr 19;257(5):1042-51. doi: 10.1006/jmbi.1996.0222.
8
Engineering the pH-optimum of activity of the GH12 family endoglucanase by site-directed mutagenesis.通过定点突变工程改造 GH12 家族内切葡聚糖酶的最适 pH 值活性。
Biochimie. 2013 Sep;95(9):1704-10. doi: 10.1016/j.biochi.2013.05.018. Epub 2013 Jun 14.
9
Glu280 is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-beta-1,4-glucanase.谷氨酸280是嗜热栖热菌CelC（一种A家族内切β-1,4-葡聚糖酶）活性位点中的亲核试剂。
J Biol Chem. 1993 Jul 5;268(19):14096-102.
10
Site-directed mutagenesis of the putative active site of endoglucanase K from Bacillus sp. KSM-330.芽孢杆菌属KSM-330内切葡聚糖酶K假定活性位点的定点诱变
Biochim Biophys Acta. 1994 Aug 17;1207(2):159-64. doi: 10.1016/0167-4838(94)00060-3.

引用本文的文献

1
His of Acetivibrio alkalicellulosi Cel5A is important for efficient hydrolysis of short cellodextrins.嗜碱纤维弧菌Cel5A的His对短纤维糊精的高效水解很重要。
AMB Express. 2025 Mar 20;15(1):53. doi: 10.1186/s13568-025-01858-w.
2
Characterization of a thermoactive endoglucanase isolated from a biogas plant metagenome.从沼气厂宏基因组中分离出的一种热活性内切葡聚糖酶的特性。
Extremophiles. 2019 Jul;23(4):479-486. doi: 10.1007/s00792-019-01099-3. Epub 2019 May 22.
3
Prediction of detailed enzyme functions and identification of specificity determining residues by random forests.
通过随机森林预测详细的酶功能和鉴定特异性决定残基。
PLoS One. 2014 Jan 8;9(1):e84623. doi: 10.1371/journal.pone.0084623. eCollection 2014.
4
Mutational and structural analyses of Caldanaerobius polysaccharolyticus Man5B reveal novel active site residues for family 5 glycoside hydrolases.对 Caldanaerobius polysaccharolyticus Man5B 的突变和结构分析揭示了家族 5 糖苷水解酶的新活性位点残基。
PLoS One. 2013 Nov 20;8(11):e80448. doi: 10.1371/journal.pone.0080448. eCollection 2013.
5
Comparative analyses of two thermophilic enzymes exhibiting both beta-1,4 mannosidic and beta-1,4 glucosidic cleavage activities from Caldanaerobius polysaccharolyticus.比较分析两种嗜热酶，它们均来自多聚糖分解栖热菌（Caldanaerobius polysaccharolyticus），同时具有β-1,4 甘露糖苷键和β-1,4 葡萄糖苷键的切割活性。
J Bacteriol. 2010 Aug;192(16):4111-21. doi: 10.1128/JB.00257-10. Epub 2010 Jun 18.
6
Characterization of Fusarium oxysporum beta-1,6-galactanase, an enzyme that hydrolyzes larch wood arabinogalactan.尖孢镰刀菌β-1,6-半乳糖苷酶的特性研究，该酶可水解落叶松木阿拉伯半乳聚糖。
Appl Environ Microbiol. 2007 May;73(9):3109-12. doi: 10.1128/AEM.02101-06. Epub 2007 Mar 9.
7
Crystal structure and snapshots along the reaction pathway of a family 51 alpha-L-arabinofuranosidase.51家族α-L-阿拉伯呋喃糖苷酶的晶体结构及反应途径中的快照
EMBO J. 2003 Oct 1;22(19):4922-32. doi: 10.1093/emboj/cdg494.
8
Structural comparisons of TIM barrel proteins suggest functional and evolutionary relationships between beta-galactosidase and other glycohydrolases.TIM桶状蛋白的结构比较表明β-半乳糖苷酶与其他糖水解酶之间存在功能和进化关系。
Protein Sci. 1999 Jan;8(1):122-36. doi: 10.1110/ps.8.1.122.
9
Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA.热纤梭菌内切葡聚糖酶CelD和纤维素体整合蛋白CipA中重复片段的识别特异性。
J Bacteriol. 1994 May;176(10):2822-7. doi: 10.1128/jb.176.10.2822-2827.1994.