Osek J, Jonson G, Svennerholm A M, Holmgren J
Department of Medical Microbiology and Immunology, University of Gothenburg, Sweden.
APMIS. 1992 Nov;100(11):1027-32. doi: 10.1111/j.1699-0463.1992.tb04036.x.
An ELISA for determination of antibodies to V. cholerae TCP was developed. Since purified TCP preparations contained detectable amounts of LPS (as shown by ELISA and immunoelectron microscopy with anti-LPS polyclonal serum), a capture ELISA was used. In this test the plate was coated with anti-TCP monoclonal antibody followed by incubation with TCP fimbriae. By this procedure no LPS bound to the solid phase as shown by the loss of reactivity with anti-LPS serum. The capture ELISA allowed sensitive and specific determination of TCP antibodies in sera of rabbits immunized with classical but not El Tor V. cholerae strains. There was good agreement between results in the TCP ELISA and reactivity with the TcpA band in immunoblot analyses when antisera raised against classical and El Tor vibrios were studied.
开发了一种用于测定霍乱弧菌毒素协同调节菌毛（TCP）抗体的酶联免疫吸附测定（ELISA）。由于纯化的TCP制剂含有可检测量的脂多糖（LPS）（如ELISA和用抗LPS多克隆血清进行的免疫电子显微镜所示），因此使用了捕获ELISA。在该试验中，平板用抗TCP单克隆抗体包被，然后与TCP菌毛一起孵育。通过该程序，如与抗LPS血清反应性丧失所示，没有LPS结合到固相上。捕获ELISA能够灵敏且特异地测定用古典生物型而非埃尔托生物型霍乱弧菌菌株免疫的兔血清中的TCP抗体。当研究针对古典生物型和埃尔托生物型弧菌产生的抗血清时，TCP ELISA结果与免疫印迹分析中TcpA条带的反应性之间具有良好的一致性。
