KOBLICK D C
J Gen Physiol. 1959 Jan 20;42(3):635-45. doi: 10.1085/jgp.42.3.635.
An enzymatic ion exchange model for active sodium transport is described. Kinetic equations relating net flux to time, and to concentration difference across the actively transporting membrane are derived. The second of these equations is tested, using the isolated frog skin in the "short-circuit" apparatus of Ussing. Reasonable linearity, as predicted by this equation, is observed. The passive permeability coefficient for Na(+), is calculated as 5.3 x 10(-4) +/- 5.3 x 10(-4) cm./hr. If cholinesterase is assumed to be the enzyme responsible for transport, the activity required to account for the observations reported here is 17.7 x 10(-4) mmoles/cm.(2)/hr.
本文描述了一种用于主动钠转运的酶促离子交换模型。推导了将净通量与时间以及与主动转运膜两侧浓度差相关的动力学方程。使用乌斯辛“短路”装置中的离体蛙皮对这些方程中的第二个进行了测试。观察到如该方程所预测的合理线性关系。计算出Na⁺的被动渗透系数为5.3×10⁻⁴±5.3×10⁻⁴厘米/小时。如果假定胆碱酯酶是负责转运的酶，那么解释此处所报告观察结果所需的活性为17.7×10⁻⁴毫摩尔/厘米²/小时。
