Ahmed K, Rikitomi N, Matsumoto K
Department of Internal Medicine, Nagasaki University, Japan.
Microbiol Immunol. 1992;36(10):1009-17. doi: 10.1111/j.1348-0421.1992.tb02105.x.
This study investigated the fimbriation on 24 fresh clinical isolates of Branhamella catarrhalis by electron microscopy. All the strains were isolated from patients with respiratory infections. The Branhamella catarrhalis strains were classified into three groups according to the grade of fimbriation. Among these 24 strains the incidence of densely fimbriated, moderately fimbriated and sparsely fimbriated isolates were 12 (50%), 7 (29%) and 5 (21%), respectively. After five-times serial subculture on Brain Heart Infusion agar, the average number of fimbriae per bacteria was decreased from 174 to 114 in the densely fimbriated strain and from 48 to 10 in the moderately fimbriated strain. Moreover, 20% of the population became non-fimbriated in moderately fimbriated strain after the serial subculture. In strains with higher hemagglutination titer the number of fimbriae was significantly (P < 0.04) more than in strains with lower hemagglutination titer.
本研究通过电子显微镜对24株来自临床的新鲜卡他布兰汉菌分离株的菌毛形成情况进行了调查。所有菌株均从呼吸道感染患者中分离得到。根据菌毛形成程度，卡他布兰汉菌菌株被分为三组。在这24株菌株中，菌毛密集型、菌毛中等型和菌毛稀疏型分离株的发生率分别为12株（50%）、7株（29%）和5株（21%）。在脑心浸液琼脂上进行5次连续传代培养后，菌毛密集型菌株中每个细菌的菌毛平均数量从174根减少到114根，菌毛中等型菌株中从48根减少到10根。此外，连续传代培养后，菌毛中等型菌株中有20%的菌苔变为无菌毛型。在血凝滴度较高的菌株中，菌毛数量显著（P<0.04）多于血凝滴度较低的菌株。
