Ahmed K
Department of Internal Medicine, Nagasaki University, Japan.
APMIS. 1992 Dec;100(12):1066-72. doi: 10.1111/j.1699-0463.1992.tb04042.x.
This study attempted to elucidate the role of fimbriae in the adherence of B. catarrhalis to human oropharyngeal epithelial cells. Antifimbrial immune serum was prepared by immunization of rabbit with whole fimbriated bacteria, and adsorption of the serum with a nonfimbriated B. catarrhalis strain. After pretreatment with the antifimbrial antiserum, the adherence of fimbriated B. catarrhalis to human epithelial cells was significantly decreased (p < 0.05). The adherence was also significantly (p < 0.001) decreased by trypsin treatment. Electron microscopy revealed destruction of fimbriae after trypsin treatment. These observations suggest that fimbriae are involved in the adherence of B. catarrhalis to epithelial cells.
本研究试图阐明菌毛在卡他莫拉菌黏附人咽上皮细胞中的作用。通过用全菌毛化细菌免疫兔子，并用非菌毛化卡他莫拉菌菌株吸附血清，制备了抗菌毛免疫血清。用抗菌毛抗血清预处理后，菌毛化卡他莫拉菌对人上皮细胞的黏附显著降低（p<0.05）。胰蛋白酶处理也使黏附显著降低（p<0.001）。电子显微镜显示胰蛋白酶处理后菌毛被破坏。这些观察结果表明菌毛参与了卡他莫拉菌对上皮细胞的黏附。
