Ferrández M A, Carrascosa A, Audí L, Ballabriga A
Department of Pediatrics, Hospital Universitario Materno-Infantil Vall d'Hebron, Autonomous University, Barcelona, Spain.
Pediatr Res. 1992 Nov;32(5):571-3. doi: 10.1203/00006450-199211000-00017.
Somatostatin effects on cultured human fetal epiphyseal chondrocytes were evaluated by studying the effects of somatostatin on DNA synthesis. Cultured epiphyseal chondrocytes from human fetuses (12-40 wk old) were incubated for 48 h in Ham's F-12 serum-free medium. After this, the medium was replaced by MCDB-104 serum-free medium and the cells were incubated for an additional 48 h in the presence or absence of somatostatin 1 pM to 10 microM, with the addition of 3H-thymidine (5 microCi/mL) for the last 24 h of incubation. A significant (p < 0.02) inhibitory effect of somatostatin (1 nM to 10 microM) on 3H-thymidine DNA incorporation was observed in cultured chondrocytes from fetuses of all gestational ages studied (12-40 wk), with no significant differences among fetal ages. In conclusion, our results show that somatostatin exerts a biologic effect on cultured human fetal epiphyseal chondrocytes, as it does in its target cells. These results suggest that somatostatin could regulate human skeletal growth not only by growth hormone secretion regulation, but also by acting directly on chondrocyte metabolism. However, the physiologic significance of the latter remains to be elucidated.
通过研究生长抑素对DNA合成的影响，评估生长抑素对培养的人胎儿骨骺软骨细胞的作用。将来自人胎儿（12 - 40周龄）的培养骨骺软骨细胞在Ham's F - 12无血清培养基中孵育48小时。之后，将培养基换成MCDB - 104无血清培养基，并在存在或不存在1 pM至10 microM生长抑素的情况下，将细胞再孵育48小时，在孵育的最后24小时加入3H - 胸腺嘧啶核苷（5微居里/毫升）。在所有研究的胎龄（12 - 40周）的胎儿培养软骨细胞中，均观察到生长抑素（1 nM至10 microM）对3H - 胸腺嘧啶核苷掺入DNA有显著（p < 0.02）抑制作用，各胎龄之间无显著差异。总之，我们的结果表明，生长抑素对培养的人胎儿骨骺软骨细胞发挥生物学作用，就如同它在其靶细胞中所起的作用一样。这些结果表明，生长抑素不仅可以通过调节生长激素分泌来调节人类骨骼生长，还可以直接作用于软骨细胞代谢。然而，后者的生理意义仍有待阐明。
