Cotte L, Escaich S, Ritter J, Martin J L, Trépo C
Unité Hépatogastroentérologie et SIDA, Hôtel-Dieu, Lyon, France.
Res Virol. 1992 Sep-Oct;143(5):329-35. doi: 10.1016/s0923-2516(06)80121-4.
To assess the correlations between clinical and biological stages of HIV infection and HIV isolation, peripheral blood mononuclear cells from 389 HIV-infected patients were studied by 30-day cocultures with normal lymphocytes. HIV isolation was successful in 279/389 patients (71.7%). Positive isolation was more frequent in CDC IV cases (82%) than in CDC II and III cases (63.6% and 78.4% respectively). There was a close correlation between culture positivity and serum beta 2-microglobulin, CD4+ cell counts and serum p24 antigen. The day of peak detection of reverse transcriptase activity or peak p24 antigen in coculture supernatants was selected as a coculture kinetic parameter. The day of peak detection of HIV in culture occurred earlier in CDC IV cases than in CDC II and III cases, and was a prognostic factor in AIDS progression at 2 years. These data suggest that in vitro parameters related to both viral burden and replicative capacity of HIV isolates are relevant indicators of disease progression.
为评估HIV感染的临床和生物学阶段与HIV分离之间的相关性，我们采用与正常淋巴细胞进行30天共培养的方法，对389例HIV感染患者的外周血单个核细胞进行了研究。389例患者中有279例（71.7%）成功分离出HIV。CDC IV期病例的阳性分离率（82%）高于CDC II期和III期病例（分别为63.6%和78.4%）。培养阳性与血清β2-微球蛋白、CD4+细胞计数及血清p24抗原之间存在密切相关性。将共培养上清液中逆转录酶活性峰值检测日或p24抗原峰值日作为共培养动力学参数。HIV在培养中峰值检测日在CDC IV期病例中比在CDC II期和III期病例中出现得更早，并且是2年时艾滋病进展的一个预后因素。这些数据表明，与HIV分离株的病毒载量和复制能力相关的体外参数是疾病进展的相关指标。
