Kuriki Y, Racker E
Biochemistry. 1976 Nov 16;15(23):4951-6. doi: 10.1021/bi00668a001.
The bioflavonoid, quercetin, inhibited the (Na+, K+)adenosine triphosphatase purified from the electric organ of electric eel (Electrophorus electricus) or from lamb kidney. An analysis of its mode of action revealed that the formation of phosphoenzyme from Pi but not from ATP was inhibited. Quercetin increased the amount of ADP-sensitive phosphoenzyme (E1--P), indicating an inhibition of the conversion of E1--P to the ADP-insensitive form (E2--P). The rate of dephosphorylation of the phosphoenzyme formed from ATP was slowed by quercetin. These results suggest that quercetin inhibits the formation of E2--P from either Pi or E1-P as well as the hydrolysis of the phosphoenzyme. Its mode of action is therefore different from that of ouabain and other inhibitors of the Na+, K+)adenosine triphosphatase.
生物类黄酮槲皮素可抑制从电鳗（电鳗属）电器官或羊肾中纯化得到的（Na +，K +）三磷酸腺苷酶。对其作用方式的分析表明，它抑制了由无机磷酸（Pi）而非三磷酸腺苷（ATP）形成磷酸化酶。槲皮素增加了对二磷酸腺苷（ADP）敏感的磷酸化酶（E1-P）的量，表明其抑制了E1-P向对ADP不敏感形式（E2-P）的转化。槲皮素减缓了由ATP形成的磷酸化酶的去磷酸化速率。这些结果表明，槲皮素抑制了由Pi或E1-P形成E2-P以及磷酸化酶的水解。因此，其作用方式不同于哇巴因和其他（Na +，K +）三磷酸腺苷酶抑制剂。
