COMBES B
Science. 1959 Feb 13;129(3346):388-9. doi: 10.1126/science.129.3346.388.
Paper chromatograms of bile collected after the intravenous administration of bromsulfalein to rats reveal four distinct bromsulfalein bands. One migrates with the same Rf as standard bromsulfalein; the other three move less fast. The major band, which accounts for 71.3 percent of the injected bromsulfalein, appears to be a conjugate of bromsulfalein with glycine and glutamic acid.