FRIEDMAN B A, CEPONIS M J
Science. 1959 Mar 13;129(3350):720-1. doi: 10.1126/science.129.3350.720-a.
Ultraviolet radiation-induced mutants of the soft rot bacterium Pseudomonas marginalis were selected for loss of pathogenicity for lettuce and witloof chicory. The avirulent mutants differed from the parent pathogen in their inability to synthesize pectolytic enzymes in culture or to ferment sodium pectate or sodium polygalacturonate as the sole carbon source in media.
选择软腐细菌边缘假单胞菌的紫外线辐射诱导突变体,使其对生菜和菊苣失去致病性。无毒突变体与亲本病原体的不同之处在于,它们在培养物中无法合成果胶分解酶,也无法在培养基中以果胶酸钠或聚半乳糖醛酸钠作为唯一碳源进行发酵。