Dittadi R, Donisi P M, Brazzale A, Marconato R, Spina M, Gion M
Center for the Study of Biological Markers of Malignancy, Regional General Hospital, Venice, Italy.
Anticancer Res. 1992 Nov-Dec;12(6B):2005-10.
The erbB2-encoded protein p185 was determined in 130 breast cancer specimens and in 29 non-malignant breast tissues using a recently available ELISA method. The assay showed good characteristics of precision and accuracy. In the non-malignant tissue p185 concentrations were normally distributed and directly correlated with estrogen receptors (ER) and progesterone receptors (PR). In cancer tissue p185 showed higher concentrations than in non-malignant tissue. No relationships were found between p185 and the main clinical and pathological parameters, except that a direct association with nuclear grade was found. We have categorized the breast cancer samples according to p185 concentrations as p185-negative (concentrations lower than the higher non-malignant tissue) and p185-positive. Our data suggested a different behaviour of p185 in samples with low or high p185 concentrations. Indeed, in p185-negative samples the concentrations were directly correlated with both ER and PR. Conversely, p185-positive samples were directly associated with node status and pT, and inversely associated with ER and PR.
采用一种最近可用的酶联免疫吸附测定（ELISA）方法，对130份乳腺癌标本和29份非恶性乳腺组织中的erbB2编码蛋白p185进行了测定。该检测方法显示出良好的精密度和准确性。在非恶性组织中，p185浓度呈正态分布，且与雌激素受体（ER）和孕激素受体（PR）直接相关。在癌组织中，p185浓度高于非恶性组织。除了发现与核分级有直接关联外，未发现p185与主要临床和病理参数之间存在相关性。我们根据p185浓度将乳腺癌样本分为p185阴性（浓度低于较高的非恶性组织）和p185阳性。我们的数据表明，p185在p185浓度低或高的样本中表现不同。实际上，在p185阴性样本中，浓度与ER和PR均直接相关。相反，p185阳性样本与淋巴结状态和pT直接相关，与ER和PR呈负相关。
