溶血性大肠杆菌的特征，特别提及1型细胞毒素坏死因子（CNF1）的产生。

Characteristics of haemolytic Escherichia coli with particular reference to production of cytotoxic necrotizing factor type 1 (CNF1).

作者信息

Blanco J, Blanco M, Alonso M P, Blanco J E, González E A, Garabal J I

机构信息

Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago, Lugo, Spain.

出版信息

Res Microbiol. 1992 Nov-Dec;143(9):869-78. doi: 10.1016/0923-2508(92)90074-x.

DOI:10.1016/0923-2508(92)90074-x

PMID:1363736

Abstract

A total of 1,106 Escherichia coli strains isolated in Spain between 1986 and 1991 from extraintestinal infections and faeces of healthy controls were examined for production of alpha-haemolysin (Hly). Among strains causing urinary tract infections, sepsis and other extraintestinal infections, Hly production was detected in 51% (P < 0.001), 32% (P < 0.001) and 18% (P < 0.02), respectively. In contrast, only 9% of faecal isolates from healthy individuals synthesized Hly. The 356 haemolytic E. coli strains characterized in this study belonged to 28 different serogroups. However, 284 (80%) were of one of eight serogroups (02, 04, 06, 08, 018, 022, 075 and 083); 40% and 31% of haemolytic strains expressed P fimbriae and mannose-resistant haemagglutination (MRHA) type III, respectively. We have found that haemolytic isolates of E. coli may clearly be divided into two categories on the basis of the ability to produce cytotoxic necrotizing factor type 1 (CNF1). The serogroups and adhesins determined in Hly+CNF1+ strains were generally different from those found in Hly+CNF1- strains. Thus, serogroups 02, 06 and 075 were associated with haemolytic E. coli producing CNF1+, whereas serogroups 01, 08, 018, 028 and 086 were established more frequently among Hly+CNF1- strains. While expression of P fimbriae was more frequently detected in Hly+CNF1- strains (70 versus 29%, P < 0.001), MRHA type III was usually identified in Hly+CNF1+ E. coli (42 versus 1%, P < 0.001). Furthermore, the sonic extracts of Hly+CNF1+ strains caused necrosis in rabbit skin (96 versus 25%, P < 0.001) and death in intraperitoneally injected mice (73 versus 11%, P < 0.001) more frequently than sonic extracts of Hly+CNF1- strains.

摘要

对1986年至1991年间在西班牙从肠道外感染患者及健康对照者粪便中分离出的1106株大肠杆菌进行了α-溶血素（Hly）产生情况的检测。在引起尿路感染、败血症及其他肠道外感染的菌株中，检测到Hly产生的比例分别为51%（P<0.001）、32%（P<0.001）和18%（P<0.02）。相比之下，健康个体粪便分离株中只有9%合成Hly。本研究中鉴定的356株溶血大肠杆菌菌株分属于28个不同血清群。然而，其中284株（80%）属于8个血清群之一（O2、O4、O6、O8、O18、O22、O75和O83）；溶血菌株中分别有40%和31%表达P菌毛和III型甘露糖抗性血凝（MRHA）。我们发现，大肠杆菌溶血分离株可根据产生1型细胞毒素坏死因子（CNF1）的能力明确分为两类。Hly+CNF1+菌株中确定的血清群和黏附素通常与Hly+CNF1-菌株中发现的不同。因此，血清群O2、O6和O75与产生CNF1+的溶血大肠杆菌相关，而血清群O1、O8、O18、O28和O86在Hly+CNF1-菌株中更常见。虽然在Hly+CNF1-菌株中更频繁检测到P菌毛表达（70%对29%，P<0.001），但III型MRHA通常在Hly+CNF1+大肠杆菌中鉴定到（42%对1%，P<0.001）。此外，Hly+CNF1+菌株的超声提取物比Hly+CNF1-菌株的超声提取物更频繁地引起兔皮肤坏死（96%对25%，P<0.001）和腹腔注射小鼠死亡（73%对11%，P<0.001）。