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抗杀虫剂按蚊的研究。2. 实验室培育的阿氏按蚊滴滴涕抗性品系的染色体排列

Studies on insecticide-resistant anophelines. 2. Chromosome arrangements in laboratory-developed DDT-resistant strains of Anopheles atroparvus.

作者信息

MOSNA E, PALMIERI C, ASCHER K R, RIVOSECCHI L, NERI I

出版信息

Bull World Health Organ. 1959;20(1):63-74.

PMID:13638790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2537789/
Abstract

The development of several DDT-resistant strains of Anopheles atroparvus is described. They were selected by larval treatment only (RL), selection of larval and adult females (RLAF), and selection of adult females and males (RAFM). Their rate of DDT-resistance was measured in the larval and adult states (mainly in the 32nd generation). The results were compared with those of a dieldrin-selected strain (R/dieldrin) and several normal and semi-normal reference strains. In the larval assay DDT-resistance was of the following order: RL>RLAF>RAFM>R/dieldrin. Topical application to adults gave: RLAF>(RL?)>RAFM>R/dieldrin; while adult tarsal contact gave: RAFM>RLAF>RL>R/dieldrin These different orders of resistance are discussed.The strains were subjected to cytological examination by the method of Frizzi. The percentages of heterozygous inversions found were as follows: R/dieldrin, 77.4%; RL, 55.3%; RLAF, 54.0%; RAFM, 24.4%; all normal and semi-normal strains about 12%-21%.The rate of DDT-resistance could not be correlated with the percentages of heterozygous inversions found in the various strains.

摘要

本文描述了几种抗滴滴涕的阿氏按蚊品系的培育情况。这些品系分别通过仅对幼虫进行处理(RL)、对幼虫和成年雌蚊进行选择(RLAF)以及对成年雌蚊和雄蚊进行选择(RAFM)的方式培育而成。在幼虫期和成虫期(主要是第32代)测定了它们对滴滴涕的抗性率。将结果与经狄氏剂选择的品系(R/狄氏剂)以及几个正常和半正常参考品系的结果进行了比较。在幼虫试验中,对滴滴涕的抗性顺序如下:RL>RLAF>RAFM>R/狄氏剂。对成虫进行局部施药时得到的顺序为:RLAF>(RL?)>RAFM>R/狄氏剂;而让成虫跗节接触滴滴涕时得到的顺序为:RAFM>RLAF>RL>R/狄氏剂。文中对这些不同的抗性顺序进行了讨论。采用弗里齐方法对这些品系进行了细胞学检查。发现的杂合倒位百分比分别如下:R/狄氏剂,77.4%;RL,55.3%;RLAF,54.0%;RAFM,24.4%;所有正常和半正常品系约为12% - 21%。对滴滴涕的抗性率与在各个品系中发现的杂合倒位百分比没有相关性。

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本文引用的文献

1
Studies on insecticide-resistant anophelines. I. Chromosome arrangements in a dieldrin-selected strain of Anopheles atroparvus.抗杀虫剂按蚊的研究。I. 狄氏剂选择品系嗜人按蚊的染色体排列
Bull World Health Organ. 1958;19(2):297-301.
2
[Experimental studies on the resistance of Anopheles to DDT dieldrin].[按蚊对滴滴涕、狄氏剂抗性的实验研究]
Z Tropenmed Parasitol. 1957 Dec;8(4):532-8.
3
Effect of DDT selection pressure on the frequency of chromosomal structures in Anopheles atroparvus.滴滴涕选择压力对嗜人按蚊染色体结构频率的影响。
Bull World Health Organ. 1957;16(4):859-64.
4
The influence of temperature and humidity upon the action of insecticides. II. Temperature during the pre-treatment period.温度和湿度对杀虫剂作用的影响。II. 预处理期间的温度。
Ann Trop Med Parasitol. 1957 Jun;51(2):194-200. doi: 10.1080/00034983.1957.11685808.
5
[Cytogenetic study of Anopheles gambiae].[冈比亚按蚊的细胞遗传学研究]
Bull World Health Organ. 1956;15(3-5):425-35.
6
Effects of age, sex and feeding on the susceptibility of mosquitoes to insecticides.年龄、性别和喂养方式对蚊子对杀虫剂易感性的影响。
Ann Trop Med Parasitol. 1956 Dec;50(4):438-43. doi: 10.1080/00034983.1956.11685786.