Tsai H W, Tsai Y F
Department of Physiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Chin J Physiol. 1992;35(4):349-56.
The effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on the norepinephrine (NE) levels of 11 areas of rat brains were investigated. Male Long-Evans rats, aged 4 weeks (young group) and 21 months (old group), were injected intraperitoneally (i.p.) with MPTP (3 mg/kg) daily for 8 days. Control rats received an i.p. injection of saline. Rats were sacrificed 24 h after the last injection. NE in various brain areas were assayed by high pressure liquid chromatography with electrochemical detection. Multidose treatment of MPTP caused no alteration of levels of NE in any brain area of young rats. In contrast, a marked increase in concentration of NE was found in locus coeruleus, dorsal raphe nuclei and amygdala of old rats. These results indicate that MPTP selectively causes lesions in the noradrenergic system of rats and that the neurotoxic effects of MPTP in rats may depend on age.
研究了1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）对大鼠脑11个区域去甲肾上腺素（NE）水平的影响。4周龄（青年组）和21月龄（老年组）的雄性Long-Evans大鼠，每天腹腔注射（i.p.）MPTP（3mg/kg），持续8天。对照大鼠腹腔注射生理盐水。在最后一次注射后24小时处死大鼠。采用高压液相色谱电化学检测法测定各脑区的NE。多剂量MPTP处理未引起青年大鼠任何脑区NE水平的改变。相反，在老年大鼠的蓝斑、中缝背核和杏仁核中发现NE浓度显著增加。这些结果表明，MPTP选择性地导致大鼠去甲肾上腺素能系统损伤，且MPTP对大鼠的神经毒性作用可能与年龄有关。
