小鼠多瘤病毒感染研究。1. 病毒定量与检测方法。
Studies of mouse polyoma virus infection. 1. Procedures for quantitation and detection of virus.
作者信息
ROWE W P, HARTLEY J W, ESTES J D, HUEBNER R J
出版信息
J Exp Med. 1959 Apr 1;109(4):379-91. doi: 10.1084/jem.109.4.379.
THREE PROCEDURES HAVE BEEN COMPARED FOR USEFULNESS IN TITRATION AND DETECTION OF POLYOMA VIRUS
production of cytopathic effect (CPE) in mouse embryo tissue culture, production of HI antibody after inoculation into weanling mice (MAP test), and production of tumors in suckling hamsters during a 3 to 5 week observation period. The tissue culture and mouse antibody production tests were generally comparable in sensitivity, reproducibility, and time required to obtain results. Titration by tumor production in suckling hamsters was not suitable for quantitation because of marked variation in susceptibility among animals. Virus was detected in tissues of normal mice from spontaneously infected colonies by either production of CPE in mouse embryo tissue culture or by the MAP test; virus was found in organs of 15 (58 per cent) of 26 mice with antibody, and 2 (8 per cent) of 24 mice without antibody.
已对三种方法在多瘤病毒滴定和检测中的实用性进行了比较
在小鼠胚胎组织培养中产生细胞病变效应（CPE）、接种到断奶小鼠后产生血凝抑制（HI）抗体（MAP试验）以及在3至5周的观察期内乳仓鼠产生肿瘤。组织培养和小鼠抗体产生试验在敏感性、可重复性和获得结果所需时间方面通常具有可比性。由于动物之间易感性存在明显差异，通过乳仓鼠肿瘤产生进行滴定不适合定量。通过在小鼠胚胎组织培养中产生CPE或通过MAP试验，在来自自发感染群体的正常小鼠组织中检测到病毒；在26只有抗体的小鼠中，15只（58%）的器官中发现了病毒，在24只无抗体的小鼠中，2只（8%）的器官中发现了病毒。
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