Szabó J, Trieb K, Gratzl R, Sztankay A, Grubeck-Loebenstein B
Department of General and Experimental Pathology, Vienna, Austria.
Acta Med Hung. 1992;49(3-4):225-31.
Human thyroid cells in culture stimulated by TSH and TSI were used in order to detect thyroglobulin expression. After three days stimulation the cells were incubated with monoclonal thyroglobulin antibody and FITC-conjugated antiglobulin. Fluorescent index (the intensity of fluorescence related to hundred analysed cells) was estimated for each experimental group. The most effective stimulation of the thyroglobulin expression was detected after TSH stimulation at the concentration of 0.1 mU/ml. TSI from active Graves' patients provoked the highest expression of thyroglobulin at concentration of 1.0 mg/ml, but the fluorescence index was lower than after TSH stimulation. The thyroglobulin expression was intracellular, large, partly confluent granules were detectable mainly in the perinuclear area. Antigen expression on the surface of cultured thyroid cells could not be detected. The morphology of thyroglobulin expression as detected by immunofluorescence, was the same after TSH and TSI stimulation. It is concluded, that both stimulating factors, i.e. TSH and TSI, are involved in the thyroglobulin expression of human thyroid cells.
为检测甲状腺球蛋白的表达，使用了在培养中受促甲状腺激素（TSH）和甲状腺刺激免疫球蛋白（TSI）刺激的人甲状腺细胞。刺激三天后，将细胞与单克隆甲状腺球蛋白抗体和异硫氰酸荧光素（FITC）偶联的抗球蛋白一起孵育。对每个实验组估算荧光指数（与一百个分析细胞相关的荧光强度）。在浓度为0.1 mU/ml的TSH刺激后，检测到甲状腺球蛋白表达的最有效刺激。来自活动性格雷夫斯病患者的TSI在浓度为1.0 mg/ml时引发了甲状腺球蛋白的最高表达，但荧光指数低于TSH刺激后。甲状腺球蛋白表达是细胞内的，主要在核周区域可检测到大的、部分融合的颗粒。未检测到培养的甲状腺细胞表面的抗原表达。通过免疫荧光检测到的甲状腺球蛋白表达的形态在TSH和TSI刺激后相同。得出结论，即两种刺激因子，即TSH和TSI，都参与人甲状腺细胞的甲状腺球蛋白表达。
