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[酶制剂质量的研究(XI)——激肽释放酶原酶制剂]

[Studies on the quality of enzyme preparations (XI)--Kallidinogenase preparations].

作者信息

Tanimoto T, Ohta M, Kawasaki N, Tanaka A

出版信息

Eisei Shikenjo Hokoku. 1990(108):20-5.

PMID:1364355
Abstract

Qualitative tests of 22 kinds of commercially available kallidinogenase preparations were carried out by the enzymological method. These preparations consisted of 16 kinds of tablets, 5 kinds of capsules and 1 kind of ampoule. One sample of tablet showed kallidinogenase activity less than 80% of the labeled amount by spectrophotometry, pH stat and kinin-liberating methods. All other preparations were found to have 90-120% 120% of the labeled amount by spectrophotometry using S-2266 as the substrate. Other enzymes, i.e. kininase, trypsin and chymotrypsin were found as impurities, but the cntants were very low.

摘要

采用酶学方法对22种市售激肽释放酶制剂进行了定性检测。这些制剂包括16种片剂、5种胶囊和1种安瓿剂。通过分光光度法、pH计法和激肽释放法,发现一种片剂样品的激肽释放酶活性低于标示量的80%。使用S-2266作为底物,通过分光光度法测定,发现所有其他制剂的活性为标示量的90%-120%。还发现有其他酶,即激肽酶、胰蛋白酶和糜蛋白酶作为杂质存在,但含量非常低。

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