Lewartowska A, Gardas A
Zakład Biochemii, Centrum Medyczne Kształcenia Podyplomowego w warszawie.
Endokrynol Pol. 1991;42(3):481-9.
An inexpensive enzyme immunoassay method was designed for the determination of thyroglobulin concentration in human blood serum. The range of concentrations of thyroglobulin which can be measured by the method is between 6 and 800 ng/ml. The sensitivity of the method is comparable to that of the commercial test kits. The values of thyroglobulin concentration obtained with the use of the described method are strongly correlated (r = 0.946) with those obtained by using the reference method (IRMA kit of Byk, Sweden). The intraassay coefficient of variation ranged from 5.5 to 10.2% and interassay coefficient of variation from 9.5 to 13.2% depending on the thyroglobulin concentration. The upper limit of blood serum thyroglobulin concentration in healthy subjects was 70 ng/ml. The results of thyroglobulin determination obtained with the described method are falsely lowered in the presence of antithyroglobulin antibodies; simultaneous determination of these antibodies is thus necessary in such a case. It seems that the described method may be used for monitoring the patients after surgical treatment of differentiated thyroid cancer aimed at early detection of metastases.
设计了一种廉价的酶免疫分析方法，用于测定人血清中的甲状腺球蛋白浓度。该方法可测量的甲状腺球蛋白浓度范围为6至800 ng/ml。该方法的灵敏度与商业检测试剂盒相当。使用所述方法获得的甲状腺球蛋白浓度值与使用参考方法（瑞典Byk公司的免疫放射分析试剂盒）获得的值高度相关（r = 0.946）。根据甲状腺球蛋白浓度的不同，批内变异系数范围为5.5%至10.2%，批间变异系数范围为9.5%至13.2%。健康受试者血清甲状腺球蛋白浓度的上限为70 ng/ml。在存在抗甲状腺球蛋白抗体的情况下，用所述方法获得的甲状腺球蛋白测定结果会被错误地降低；因此，在这种情况下必须同时测定这些抗体。看来，所述方法可用于监测分化型甲状腺癌手术治疗后的患者，以早期发现转移灶。
