血管活性肠肽（VIP）对大鼠胃平滑肌收缩反应的影响。
Effects of vasoactive intestinal peptide (VIP) on contractile responses of smooth muscle in rat stomach.
作者信息
Ohta T, Ito S, Ohga A
机构信息
Department of Pharmacology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
出版信息
Br J Pharmacol. 1991 Mar;102(3):621-6. doi: 10.1111/j.1476-5381.1991.tb12222.x.
Abstract
- The effects of vasoactive intestinal peptide (VIP) on contractile responses to carbachol (CCh), KCl and caffeine of the circular smooth muscle in rat stomach were examined by the isometric tension recording method and by measurement of the intracellular Ca level, [Ca]i, with fura 2. 2. Removal of extracellular Ca or nifedipine (0.1 microM) inhibited contractions induced by KCl (40 mM) and a low concentration (1 microM) of CCh but not that induced by caffeine (3 mM). After these treatments, the contraction induced by a high concentration of CCh (100 microM) changed to a phasic response. 3. VIP dose-dependently inhibited the contraction induced by 1 microM CCh, but not those caused by 40 mM KCl or 3 mM caffeine. 4. In Ca-free solution containing 2 mM EGTA, VIP inhibited the phasic contraction induced by 100 microM CCh, but not that induced by 30 mM caffeine. 5. CCh caused dose-dependent tension development concomitant with the increase in [Ca]i. VIP reduced both responses and thus did not affect the [Ca]i-force relation for CCh. In the chemically skinned muscle fibres, VIP had no effect on the pCa-tension relation. 6. It is suggested that the inhibitory effects of VIP on CCh-induced contractions are due to the inhibition of the processes of signal transduction from muscarinic receptors to voltage-dependent Ca channels and to intracellular Ca stores.
摘要
- 采用等长张力记录法并运用fura 2测量细胞内钙水平[Ca]i，研究了血管活性肠肽（VIP）对大鼠胃环形平滑肌对卡巴胆碱（CCh）、氯化钾（KCl）和咖啡因收缩反应的影响。2. 去除细胞外钙或使用硝苯地平（0.1微摩尔）可抑制由40毫摩尔KCl和低浓度（1微摩尔）CCh诱导的收缩，但不影响由3毫摩尔咖啡因诱导的收缩。经过这些处理后，由高浓度CCh（100微摩尔）诱导的收缩转变为相位反应。3. VIP剂量依赖性地抑制由1微摩尔CCh诱导的收缩，但不影响由40毫摩尔KCl或3毫摩尔咖啡因引起的收缩。4. 在含有2毫摩尔乙二醇双四乙酸（EGTA）的无钙溶液中，VIP抑制由100微摩尔CCh诱导的相位收缩，但不影响由30毫摩尔咖啡因诱导的收缩。5. CCh引起剂量依赖性的张力发展，同时伴随着[Ca]i的增加。VIP降低了这两种反应，因此不影响CCh的[Ca]i-力关系。在化学去膜的肌纤维中，VIP对pCa-张力关系没有影响。6. 提示VIP对CCh诱导收缩的抑制作用是由于抑制了从毒蕈碱受体到电压依赖性钙通道以及细胞内钙储存的信号转导过程。
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