Imayama S, Furumura M, Hori Y
Department of Dermatology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Exp Dermatol. 1992 Oct;1(3):134-40. doi: 10.1111/j.1600-0625.1992.tb00005.x.
Scanning electron microscopy with immunogold labeling revealed that epidermal keratinocytes expressed ICAM-1 (intercellular adhesion molecule-1) and HLA-DR molecules on their surfaces in patterns that differed in mycosis fungoides (MF) and lichenoid reaction (LR). ICAM-1 molecules, visualized as deposits of gold particle, were visualized as clusters adjacent to the junctions interconnecting the keratinocytes of MF lesions. LFA-1 (leukocyte function-associated antigen-1) molecules were seen as granules on the surfaces of all infiltrates, most of which also expressed ICAM-1. HLA-DR molecules were seen continuously along the borders of the individual keratinocytes, thus producing a cobblestone appearance on the epidermal undersurface. In contrast, ICAM-1 and HLA-DR were found only sparsely on the undersurface of the epidermis from LR. These findings may help to explain the differing histological features of MF and LR: ICAM-1 molecules present on the intercellular junctions of MF epidermis lead the LFA-1-bearing cells to migrate into the interspaces, thus producing epidermotropism. These cells aggregate by means of co-expressed ICAM-1 to thus produce Pautrier's microabscess. In LR, the minimal expression of ICAm-1 on the epidermal undersurface leaves most infiltrates within the dermis, thus producing a band-like infiltrate.
免疫金标记扫描电子显微镜显示，表皮角质形成细胞在其表面表达细胞间黏附分子-1（ICAM-1）和HLA-DR分子，其模式在蕈样肉芽肿（MF）和苔藓样反应（LR）中有所不同。ICAM-1分子以金颗粒沉积物的形式可见，在MF病变中，ICAM-1分子以金颗粒沉积物的形式可见，呈簇状分布在连接角质形成细胞的连接处附近。淋巴细胞功能相关抗原-1（LFA-1）分子在所有浸润细胞表面呈颗粒状可见，其中大多数也表达ICAM-1。HLA-DR分子沿单个角质形成细胞的边界连续可见，因此在表皮下表面呈现鹅卵石样外观。相比之下，在LR的表皮下表面仅稀疏发现ICAM-1和HLA-DR。这些发现可能有助于解释MF和LR不同的组织学特征：MF表皮细胞间连接处存在的ICAM-1分子引导携带LFA-1的细胞迁移到细胞间隙，从而产生亲表皮现象。这些细胞通过共同表达的ICAM-1聚集，从而形成帕蒂埃微脓肿。在LR中，表皮下表面ICAm-1的最小表达使大多数浸润细胞留在真皮内，从而产生带状浸润。
