Adrian G S, Fischbach K, Lu Y, Gayet O, Rivera E, Bowman B H
Department of Cellular & Structural Biology, University of Texas Health Science Center, San Antonio 78284.
SAAS Bull Biochem Biotechnol. 1990 Jan;3:97-101.
The iron-binding plasma protein transferrin (TF) is essential for supplying iron to cells and the prevention of iron toxicity. Our laboratory has cloned and characterized the human TF gene. Comparison of promoter regions of TF genes from human, chicken, and mouse reveals a strong nucleotide sequence conservation. This study demonstrates that 5' flanking regions of the TF gene are sufficient for directing expression of a heterologous gene in transgenic mice and transfected cells. For cell-specific expression, more than 150 base pairs appear to be required.
铁结合血浆蛋白转铁蛋白（TF）对于向细胞供应铁及预防铁中毒至关重要。我们实验室已克隆并鉴定了人类TF基因。对人类、鸡和小鼠TF基因启动子区域的比较显示出强烈的核苷酸序列保守性。这项研究表明，TF基因的5'侧翼区域足以在转基因小鼠和转染细胞中指导异源基因的表达。对于细胞特异性表达，似乎需要超过150个碱基对。
