Godia F, Adler H I, Scott C D, Davison B H
Unitat d'Enginyeria Quimica, Universitat Autonoma de Barcelona, Spain.
Biotechnol Prog. 1990 May-Jun;6(3):210-3. doi: 10.1021/bp00003a009.
Oxygen-reducing membrane fragments obtained from Escherichia coli were used with Clostridium acetobutylicum (C. acetobutylicum) to provide an oxygen-free microenvironment for the conversion of glucose to acetone, butanol, and ethanol (ABE). The batch fermentation of suspended C. acetobutylicum NRRL-B-643 and its ability to produce solvents in the presence of membranes as the oxygen-elimination agent are described and compared with the conventional sparging technique used to maintain anaerobiosis. The use of membrane fragments to remove oxygen for fermentation by C. acetobutylicum was successful and gave slightly improved results over the use of sparing with regard to lag, biomass, and solvent production (e.g., final butanol concentration of 3.25 and 2.7 g/L, respectively). Solvent production is also reported for a continuous columnar reactor with coimmobilized cells and membranes in kappa-carrageenan gel beads and air-saturated liquid feed.
从大肠杆菌获得的脱氧膜片段与丙酮丁醇梭菌一起使用，为将葡萄糖转化为丙酮、丁醇和乙醇（ABE）提供无氧微环境。描述了悬浮的丙酮丁醇梭菌NRRL-B-643的分批发酵及其在作为除氧剂的膜存在下产生溶剂的能力，并与用于维持厌氧状态的传统鼓泡技术进行了比较。使用膜片段为丙酮丁醇梭菌发酵去除氧气是成功的，并且在延迟期、生物量和溶剂生产方面比使用鼓泡略有改善（例如，最终丁醇浓度分别为3.25和2.7 g/L）。还报道了在κ-卡拉胶凝胶珠中细胞和膜共固定且进料为空气饱和液体的连续柱状反应器中的溶剂生产情况。
