利用DNA修复缺陷型大肠杆菌菌株通过电阻抗快速检测直接作用的DNA诱变剂。
The rapid detection of direct-acting DNA mutagens by electrical impedence with a DNA repair-deficient strain of Escherichia coli.
作者信息
Forsythe S J
机构信息
Division of Microbiology and Genetics, Polytechnic of East London, UK.
出版信息
Lett Appl Microbiol. 1990 Jul;11(1):30-2. doi: 10.1111/j.1472-765x.1990.tb00129.x.
A differential killing assay using Escherichia coli WP2 (wild type) and WP67 (uvrA, polA) was combined with impedence microbiology to produce a rapid screening method for direct-acting mutagenic compounds. The assay showed that mitomycin C, N-nitroso guanidine, potassium dichromate, sodium azide and acridine orange were direct-acting mutagens. With this method results can be obtained within hours, as compared with two days for the standard Salmonella/microsome test.
采用大肠杆菌WP2（野生型）和WP67（uvrA、polA）进行的差异杀伤试验与阻抗微生物学相结合，产生了一种用于直接作用诱变化合物的快速筛选方法。该试验表明，丝裂霉素C、N-亚硝基胍、重铬酸钾、叠氮化钠和吖啶橙是直接作用诱变剂。使用这种方法，数小时内即可获得结果，而标准的沙门氏菌/微粒体试验则需要两天时间。
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