二异丙基氟磷酸酯对巯基蛋白酶的作用。
Effect of diisopropylfluorophosphate on sulfhydryl proteases.
作者信息
HEINICKE R M, MORI R
出版信息
Science. 1959 Jun 19;129(3364):1678. doi: 10.1126/science.129.3364.1678.
Abstract
Diisopropylfluorophosphate inhibited all the sulfhydryl proteases studied in our tests. This inhibition was most pronounced at pH 6.0. By first blocking the sulfhydryl group with p-chloromercuribenzoate, inhibition could be prevented. Neither cysteine nor choline gave appreciable reactivation of diisopropylfluorophosphate-inhibited bromelain.
摘要
二异丙基氟磷酸酯在我们的测试中抑制了所有研究的巯基蛋白酶。这种抑制在pH 6.0时最为明显。通过先用对氯汞苯甲酸封闭巯基，可以防止抑制作用。半胱氨酸和胆碱都不能使被二异丙基氟磷酸酯抑制的菠萝蛋白酶有明显的复活。
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