用于培养产生单克隆抗体的杂交瘤细胞的盘管式膜生物反应器。

Coiled tube membrane bioreactor for cultivation of hybridoma cells producing monoclonal antibodies.

作者信息

Hagedorn J, Kargi F

机构信息

Department of Chemical Engineering, Washington University, St. Louis, Missouri 63130.

出版信息

Enzyme Microb Technol. 1990 Nov;12(11):824-9. doi: 10.1016/0141-0229(90)90017-k.

DOI:10.1016/0141-0229(90)90017-k

PMID:1366861

Abstract

A coiled tube membrane reactor was developed for the cultivation of mouse-mouse hybridoma cells producing monoclonal antibodies. The cell and antibody concentrations obtained in the membrane reactor were higher than that obtained in a batch spinner flask without a membrane. A mathematical model has been developed to describe the membrane transport coupled growth and product formation, and the physical and kinetic constants of the system were determined.

摘要

开发了一种用于培养产生单克隆抗体的小鼠-小鼠杂交瘤细胞的盘管膜生物反应器。膜生物反应器中获得的细胞和抗体浓度高于无膜的分批搅拌瓶中获得的浓度。已建立一个数学模型来描述膜传输耦合生长和产物形成，并确定了该系统的物理和动力学常数。