Coco-Martin J M, Martens D E, van der Velden-de Groot T A, Beuvery E C
The Netherlands Cancer Institute, Division of Experimental Therapy, Amsterdam.
Cytotechnology. 1993;13(3):213-20. doi: 10.1007/BF00749817.
The stability of the hybridoma cell line MN12 in a long-term homogeneous continuous culture was studied using a panel of analytical methods. These include two flow cytometry methods, for the determination of relative cytoplasmic and membrane IgG content. In addition, the antibody production was determined by an ELISA, and the metabolic state of the cells was determined by means of glucose consumption and lactate production. These results indicate a possible selection of variants of MN12 hybridoma cells with an overall aerobic metabolism, but with a higher glucose consumption rate and a higher lactate production rate. These variants are mainly characterized by a different membrane IgG content and cytoplasmic antibody content. These changes may possibly be affected by the culture age.
使用一系列分析方法研究了杂交瘤细胞系MN12在长期均匀连续培养中的稳定性。这些方法包括两种流式细胞术方法，用于测定相对细胞质和膜IgG含量。此外，通过ELISA测定抗体产量，并通过葡萄糖消耗和乳酸产生来确定细胞的代谢状态。这些结果表明可能选择了具有总体有氧代谢，但葡萄糖消耗率较高和乳酸产生率较高的MN12杂交瘤细胞变体。这些变体的主要特征是膜IgG含量和细胞质抗体含量不同。这些变化可能受培养时间的影响。
