Ryll T, Lucki-Lange M, Jäger V, Wagner R
Gesellschaft für Biotechnologische Forschung, Arbeitsgruppe Zellkulturtechnik, Braunschweig, F.R.G.
J Biotechnol. 1990 Jun;14(3-4):377-92. doi: 10.1016/0168-1656(90)90120-z.
For the production of recombinant human interleukin-2 (IL-2) two different culture processes, a 1-2 liter homogeneous stirred bubble-free aerated system and a dense cell hollow fibre bioreactor were compared. Cultivations were carried out with serum- or protein-free medium formulations. In the stirred culture 0.75 mg IL-2 were produced with 1 l of perfused medium at a maximum cell number of 3 X 10(10). The product yield in the hollow fibre module was only 0.23 mg l-1 at a maximum cell number of 6 X 10(10). In contrast to results with hybridoma or EBV-transformed cell lines, in which hollow fibre bioreactors showed comparable efficiency to perfused stirred tank reactors, the tissue-like cell density is disadvantageous as adherent cells tend to stick together leaving insufficient intercellular space for removal of product.
为生产重组人白细胞介素-2（IL-2），对两种不同的培养工艺进行了比较，一种是1-2升的均匀搅拌无泡通气系统，另一种是高密度细胞中空纤维生物反应器。培养采用无血清或无蛋白培养基配方。在搅拌培养中，1升灌注培养基在最大细胞数为3×10¹⁰时产生了0.75毫克IL-2。中空纤维模块中的产物产量在最大细胞数为6×10¹⁰时仅为0.23毫克/升。与杂交瘤或EBV转化细胞系的结果不同，在杂交瘤或EBV转化细胞系中，中空纤维生物反应器显示出与灌注搅拌罐反应器相当的效率，而组织样细胞密度是不利的，因为贴壁细胞往往会粘在一起，没有足够的细胞间隙用于产物去除。
