Engelhart W G
CEM Corp., Matthews, North Carolina.
Am Biotechnol Lab. 1990 Nov;8(15):30, 32, 34.
The speed, accuracy, and convenience of microwave hydrolysis make it a promising new technique in the protein chemistry field. Acid hydrolysis of protein samples has traditionally been the rate limiting step in amino acid analysis. Complete hydrolysis and analysis of proteins and synthetic peptides is now possible in less than an hour using microwave hydrolysis. This represents a significant improvement in quality control monitoring capability during the synthesis of high purity peptides. Microwave hydrolysis will also facilitate basic research in molecular biology and streamline sample preparation procedures for amino acid assays in the food science area.
微波水解的速度、准确性和便利性使其成为蛋白质化学领域一项很有前景的新技术。传统上，蛋白质样品的酸水解一直是氨基酸分析中的限速步骤。现在，使用微波水解技术，在不到一小时的时间内就可以完成蛋白质和合成肽的完全水解及分析。这代表着在高纯度肽合成过程中的质量控制监测能力有了显著提高。微波水解还将促进分子生物学的基础研究，并简化食品科学领域氨基酸测定的样品制备程序。
