用于检测生物技术产品生产中所用细胞培养物中潜在病毒的方法。
Methods for the detection of adventitious viruses in cell cultures used in the production of biotechnology products.
作者信息
Poiley J A
机构信息
Hazleton Laboratories America, Inc., Kensington, Maryland.
出版信息
Bioprocess Technol. 1990;10:483-94.
PMID:1367069
Abstract
The possibility for viral contamination exists in established cultures as well as in primary cultures. The use of established genetically engineered cultures in the production of biologicals for human use requires that these cultures be monitored for adventitious viral agents. Among the methods used for this purpose are animal inoculation and in vitro assays which provide a broad-spectrum screen for viral agents.
摘要
在已建立的细胞培养物以及原代培养物中都存在病毒污染的可能性。在生产供人类使用的生物制品时使用已建立的基因工程细胞培养物，需要对这些培养物进行检测，以查找是否存在外来病毒因子。用于此目的的方法包括动物接种和体外检测，这些方法可为病毒因子提供广谱筛查。
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