GOLDSTEIN L, MICOU J
J Biophys Biochem Cytol. 1959 Aug;6(1):1-6. doi: 10.1083/jcb.6.1.1.
The movement of ribonucleic acid (RNA) from nucleus to cytoplasm has been studied, by autoradiographic techniques, in cells of the human amnion grown in tissue culture. Cells were exposed to cytidine-H(3) for 1 hour after which time only the RNA of the nuclei was labelled. After this 1 hour exposure the cells were placed in a medium containing an excess amount of unlabelled cytidine. Periodically, cells from this medium were fixed. Autoradiographs showed that there was a progressive movement of the label from nucleus to cytoplasm, such that after 24 hours essentially all the label was in the RNA of the cytoplasm. A study of the incorporation of the cytidine-H(3) in deoxyribonucleic acid (DNA), in the same population of cells at the same times, indicated that the presence of excess amounts of unlabelled cytidine almost instantaneously inhibited further utilization of cytidine-H(3). It is concluded that RNA moves from nucleus to cytoplasm as a complex polynucleotide structure.
运用放射自显影技术，对组织培养的人羊膜细胞中核糖核酸（RNA）从细胞核向细胞质的移动进行了研究。细胞在胞嘧啶-H(3)中暴露1小时，此时只有细胞核的RNA被标记。在这1小时的暴露之后，将细胞置于含有过量未标记胞嘧啶的培养基中。定期对该培养基中的细胞进行固定。放射自显影片显示，标记物从细胞核向细胞质有一个渐进的移动过程，以至于在24小时后，基本上所有的标记物都存在于细胞质的RNA中。在同一时间对同一群细胞中胞嘧啶-H(3)掺入脱氧核糖核酸（DNA）的情况进行的一项研究表明，过量未标记胞嘧啶的存在几乎立即抑制了胞嘧啶-H(3)的进一步利用。得出的结论是，RNA作为一种复杂的多核苷酸结构从细胞核移动到细胞质。
