用于乳糖检测的基于安培法的双酶生物传感器——表征与应用
Amperometric bi-enzyme based biosensor for the detection of lactose--characterization and application.
作者信息
Pfeiffer D, Ralis E V, Makower A, Scheller F W
机构信息
Academy of Sciences of the GDR, Central Institute of Molecular Biology, Berlin-Buch.
出版信息
J Chem Technol Biotechnol. 1990;49(3):255-65. doi: 10.1002/jctb.280490307.
Based on the glucose oxidase-beta-galactosidase sequence an enzyme probe for the specific determination of lactose has been developed. beta-Galactosidases from different sources have been compared, the sensor containing beta-galactosidase from Curvularia inaequalis has been characterized in respect of optimal pH, enzyme loading, apparent activity and functional stability. The response of the bi-enzyme probe depends linearly on lactose concentration between 0.02 and 3.00 mmol dm-3. The application to different milk and foodstuff samples resulted in good correlations toward enzymatic photometric (y = (0.956x-1.67) mmol dm-3) and infrared detection (y = (1.0772x-0.3909)%). Using a measuring frequency of 100 h-1 the serial imprecision is about 2% for diluted milk, urine, or foodstuff samples.
基于葡萄糖氧化酶 - β - 半乳糖苷酶序列，已开发出一种用于特异性测定乳糖的酶探针。对来自不同来源的β - 半乳糖苷酶进行了比较，对含有不等弯孢霉β - 半乳糖苷酶的传感器在最佳pH值、酶负载量、表观活性和功能稳定性方面进行了表征。双酶探针的响应在0.02至3.00 mmol dm⁻³的乳糖浓度范围内呈线性关系。将其应用于不同的牛奶和食品样品，与酶光度法（y = (0.956x - 1.67) mmol dm⁻³）和红外检测（y = (1.0772x - 0.3909)%）具有良好的相关性。对于稀释的牛奶、尿液或食品样品，使用100 h⁻¹的测量频率时，连续不精密度约为2%。
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